Eos 70d camera

Both specimens of Lechytianovaezealandiae sp. nov. examined for this study had been preserved in 75% ethanol. They were studied as temporary slide mounts, prepared by immersing of the specimens in lactic acid for clearing. After the study, they were rinsed in water and returned to 75% ethanol, with the dissected portions being placed in microvials.
Morphological and morphometric analyses were performed using a Leica DM1000 compound microscope with an ICC50 Camera Module (LAS EZ application, 1.8.0). Measurements were taken from digital images (photographed using a Leica DM2500 compound microscope with a Canon EOS 70D camera) using the AxioVision 40LE application. Reference points for measurements follow Chamberlin (1931) . Drawings were generated using a Leica DM1000 drawing tube. Digital photograph of the new species was taken using a Canon EOS 5D camera attached to a Zeiss Axio Zoom V16 stereomicroscope. Image stacks were produced manually, combined using the Zerene Stacker software and edited with Adobe Photoshop CC.
The terminology follows Harvey (1992) (link), except for the use of the terms rallum (Judson 2007 (link)) and duplex trichobothria (Judson 2018 (link)).
The types of the new species are deposited in the zoological collection of the Museum of New Zealand Te Papa Tongarewa, New Zealand.

To determine the fungal burden within the larvae, larvae were infected and treated as described above. At 24 and 72 hours post-infection, five larvae from each group were sacrificed for histological examination and assessment of fungal burden. The larvae were fixed in 10% buffered formalin for 24 hours, dissected longitudinally into two halves with a scalpel and fixed for another 48 hours before further routine histological processing [20 (link)]. The two halves were stained with hematoxylin and eosin (HE) and Grocott methanamine silver for further histological examination. Assessment of the fungal burden was performed by manually counting of the grains by three independent scientists, under a light microscope mounted with a Canon EOS70D camera (Canon Inc). Visualization of the grains was done on the computer screen using EOS Utility software (Canon Inc.). Under 40x magnification with a light microscope, the grains were categorized into large (>0.02mm²), medium (0.01–0.019 mm²) and small (0.005–0.009 mm²) sizes as described by Lim et al. [21 (link)]. The sum of all large, medium and small grains represents the total amount of grains observed within the larvae. In order to determine the total size of the grains, the sum of the all grains were multiplied by the minimum size of their respected categories [21 (link)].

The agrobacterium cultures carrying the pCHF3 empty vector, 35S-GFP, 35S-P19 and 35S-P0 were prepared, and the final OD600 was adjusted to 1.0. The following groups of empty vector + 35S-GFP, 35S-P0 + 35S-GFP and 35S-P19 + 35S-GFP were mixed according to a 5:4 ratio and inoculated into the seedlings of transgenic 16c N. benthamiana plants carrying the GFP gene. GFP fluorescence was detected under a hand-held 100W, long-wave UV lamp (UV products, Upland, CA, USA) and photographed using a Canon EOS 70D camera.

The pathological changes in AS were detected with en face oil-red O staining, HE staining, and Masson's trichrome staining (n = 5/group) (Details in Supplementary Material). Images were captured with Canon EOS 70D camera and were analyzed using Image J 1.8.0 software (National Institutes of Health, United States). The lesion area index was calculated as the percentage of aortic lumen area covered by atherosclerotic lesions. Observers were blinded to the experimental groups.

Transgenic roots were photographed under the dissecting fluorescent microscope with a Canon EOS 70D camera (Canon, Japan) and the intensity values (arbitrary units) of YFP fluorescence quantified by Image J software (NIH, Bethesda, MD; http://rsb.info.nih.gov/ij).
YFP intensity was determined by subtracting the background Region of Interest (ROI) adjacent to the YFP root ROI. Similar ROI calculations were done at different time points to quantitate YFP changes over time. The temporal changes in YFP fluorescent intensities were expressed relative to the basal YFP intensity prior to treatment.
Transformed roots from pPopY-hpYFP or pER8Y-hpYFP system were induced by indicated concentrations of Dex or estradiol respectively and photographed for YFP fluorescence daily. Reversibility of induction was investigated by transferring pPopY-hpYFP roots onto media without Dex or with 50 uM Dex and photographed an additional 16 days.

Unless otherwise noted, ink was removed by hexane wiping prior to the measurements. All thermal images were obtained with a Fluke Ti10 thermal imager. All polarized optical images were taken using a Canon EOS 70D camera (ISO 1250, shutter speed 1/125, aperture f/4) equipped with a polarizer. A white light-emitting diode (LED) screen was used as the polarized background light. Samples were typically placed between the LED screen and the polarizer in a direction that inclined to the polarization direction of the background light at an angle of 45°. Color spectra were collected using an optical spectrometer (USB2000 UV-VIS, Ocean Optics company). Light intensity at any given wavelength within the measurement range can be extracted from a spectrum. Collecting multiple color spectra during the course of sample stretching or iso-strain stress relaxation allowed monitoring the change in light intensity for a given wavelength.