Tris, glucose, citric acid and glycerol were purchased from Sangon Biotech (Shanghai, China). Penicillin–streptomycin was purchased from Thermo Fisher (Waltham, Massachusetts, USA). The freezing extender I contained Tris base 300.48 mM, glucose 27.75 mM, citric acid 94.72 mM and Penicillin–streptomycin 200 IU/mL with 20% egg yolk. The freezing extender II contained 94% freezing extender I and 6% glycerol. Peanut agglutinin conjugated with fluorescein isothiocyanate (FITC‐PNA) was purchased from Sigma (St Louis, MO, USA). Propidine iodide (PI) was purchased from Solarbio (Beijing, China). ROS assay kit was purchased from Beyotime (Shanghai, China).
Propidine iodide pi
Manufactured by Solarbio
Sourced in China
Propidine iodide (PI) is a fluorescent dye used in various laboratory applications. It is a DNA-binding agent that emits red fluorescence upon binding to nucleic acids. PI is commonly used for cell viability and cell cycle analysis through flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Ros assay kit, by Beyotime (1 mentions)
Citric acid, by Sangon (1 mentions)
Glycerol, by Sangon (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc, by Solarbio (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by Vazyme (1 mentions)
Flow cytometry, by BD (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Solarbio (1 mentions)
6 protocols using propidine iodide pi
1
Semen Cryopreservation Protocol
2
Quantifying Cell Apoptosis via Flow Cytometry
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Cell apoptosis was measured by using Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit (Invitrogen). PC cells were harvested after transfection for 72 h through centrifuging at 800 ×g, and the above cells were mixed with Annexin V-FITC and propidine iodide (PI; Solarbio, Beijing, China) simultaneously at room temperature in the dark for 10 min. The apoptotic PC cells were distinguished from necrotic or normal PC cells through the analysis of flow cytometer (BD Biosciences, San Jose, CA, USA).
3
Cell Cycle Analysis of NSCLC
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NSCLC cells were collected and fixed using 70% cold ethanol solution at −20°C overnight. Then, 10 μM RNase was utilized to remove RNA. DNA was marked with 20 mg/mL propidine iodide (PI; Solarbio, Beijing, China) for 20 minutes at 37°C. The percentage of NSCLC cells at different phases was assessed using a flow cytometer (BD Biosciences, San Jose, CA, USA).
4
Cell Cycle and Apoptosis Analysis
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For cycle distribution detection, transduced A549/PTX and H1299/PTX cells were gathered and re‐suspended in PBS following 48 h of transfection. After RNase treatment, A549/PTX and H1299/PTX cells were immobilized with 70% ethanol followed by the incubation of propidine iodide (PI; Solarbio) at 37°C for 30 min. Then, the cycle distribution of A549/PTX and H1299/PTX cells at different phases was examined by flow cytometry (BD Biosciences).
Annexin V‐fluorescein isothiocyanate (FITC) Apoptosis Detection Kit (Vazyme) was utilized for detecting cell apoptosis. After different transfection for 48 h, A549/PTX and H1299/PTX cells were harvested and double‐stained with 5 μL of FITC‐labeled Annexin V and 5 μL of PI. The apoptotic cells were measured utilizing flow cytometry (BD Biosciences).
Annexin V‐fluorescein isothiocyanate (FITC) Apoptosis Detection Kit (Vazyme) was utilized for detecting cell apoptosis. After different transfection for 48 h, A549/PTX and H1299/PTX cells were harvested and double‐stained with 5 μL of FITC‐labeled Annexin V and 5 μL of PI. The apoptotic cells were measured utilizing flow cytometry (BD Biosciences).
5
Cell Cycle and Apoptosis Analysis
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Flow cytometry was used for the detection of cell cycle distribution and cell apoptosis. After being transfected for 48 h, KYSE‐150 and Eca‐109 cells were harvested. For the determination of cell cycle distribution, transduced KYSE‐150 and Eca‐109 cells were gathered and washed with phosphate buffer solution (PBS), followed by immobilization with 70% ethanol at 4°C overnight. Then the cells were resuspended in binding buffer and dyed with propidine iodide (PI; Solarbio) in the dark at 37°C for 30 min. Finally, the distributed proportions of KYSE‐150 and Eca‐109 cells at different phases (G0/G1, S, and G2) were monitored through a flow cytometer.
An Annexin V‐FITC/PI Apoptosis Detection kit (Solarbio) was utilized for apoptotic detection. Briefly, transfected KYSE‐150 and Eca‐109 cells were dyed using Annexin V‐FITC and propidium iodide (PI) in darkness for 15 min. The apoptotic rate of KYSE‐150 and Eca‐109 cells (Annexin V‐FITC+ and PI+ or PI−) was measured using a flow cytometer.
An Annexin V‐FITC/PI Apoptosis Detection kit (Solarbio) was utilized for apoptotic detection. Briefly, transfected KYSE‐150 and Eca‐109 cells were dyed using Annexin V‐FITC and propidium iodide (PI) in darkness for 15 min. The apoptotic rate of KYSE‐150 and Eca‐109 cells (Annexin V‐FITC+ and PI+ or PI−) was measured using a flow cytometer.
6
Cell Cycle Analysis of CRC Cells
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CRC cells were harvested with cold phosphate buffer saline (PBS), and fixed in 70% cold ethanol solution (diluted with PBS) at − 20 °C overnight. After incubating with RNase (10 μM) for 30 min, propidine iodide (PI; 20 mg/mL; Solarbio, Beijing, China) was used to stain CRC cells for 20 min at 37 °C. The cell cycle was evaluated on a flow cytometer (BD Biosciences, San Jose, CA, USA).
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