Pipette gun

As the Chinese alligator is an endangered species, in order to preserve the finite amount of serum, the experiment was designed as follows. Briefly, we took 96-well culture plates, zoned, and marked each plate. For each plate, 100 μL MHB was pipetted to wells 2–11 via a pipette gun (Eppendorf, Hamburg, Germany), and 200 μL MHB was pipetted into well 12. Then 200 μL P/S or serum was pipetted into well 1, and 100 μL of the respective treatment was pipetted to well 2. The liquid was mixed adequately with a pipette gun to suck it up and blow it back, and this step was repeated 3–5 times. Then 100 μL was removed from well 2 to and pipetted into well 3, this operation was repeated, until reaching well 10. The 100 μL solution in well 10 was not mixed in this manner and remained unchanged. Then, we pipetted 100 μL of the bacteria solution into wells 1–11 (Figure S5). Following culturing at 37 °C for 18 h, spectrophotometry of the solution was conducted with SynergyMx M5 Full-band Multifunctional Microplate reader (Molecular Devices, San Jose, CA, USA). This experiment was repeated three times.

A total of 40 ml of arterial blood was drawn from each patient and randomly injected into 10 blood collection tubes (4 ml/tube), centrifuged, then the serum was aspirated to prepare the blood clot model, and divided into the standard group, control group, 0.125 mg/3ml, 0.25 mg/3ml, 0.5 mg/3ml, 1 mg/3ml, 2 mg/3ml, 3 mg/3ml rt-PA groups, 20,000 U, and 40,000 U urokinase (u-PA) groups. No intervention in the standard group for checking blood clot volume, the control group was added with 3 ml saline, then 0.125 mg/3 ml, 0.25 mg/3 ml, 0.5 mg/3 ml, 1 mg/3 ml, 2 mg/3 ml, 3 mg/3 ml rt-PA groups were added with the corresponding dose of the rt-PA (Boehringer-Ingelheim, Germany), and the 20,000 and 40,000 U u-PA groups were added with the corresponding dose of the u-PA (NDPHARM, China). Then, the tube was placed in a 37°C electric-heated thermostatic water bath (Wuxi Yierda, China), and the corresponding dissolved volume or blood clot volume was measured 30, 60, 90, 120, and 150 min after the intervention with a pipette gun (Eppendorf, Germany).