The following antibodies were used for Western-blotting: β-actin (A2066; Sigma), gB (6506; Abcam), p-ERK (4370; cell signaling) and p-IκBα (9246; Cell Signaling). Mitoxantrone dihydrochloride was purchased from sigma (M6545). HSV-1 ICP6 polyclonal antibody was generated against the N-terminal peptide of ICP6. TNF-α recombinant protein was generated according to previously instruction [38 (link)].
Mitoxantrone dihydrochloride
Manufactured by Merck Group
Sourced in Germany, United States
Mitoxantrone dihydrochloride is a synthetic anthraquinone derivative used as a laboratory reagent. It is a DNA intercalating agent with antineoplastic and immunosuppressive properties. The compound can be used in various in vitro and in vivo research applications.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Malvidin chloride, by Merck Group (1 mentions)
Pelargonidin chloride, by Merck Group (1 mentions)
Natdc, by Merck Group (1 mentions)
16 dsa, by Merck Group (1 mentions)
5 dsa, by Merck Group (1 mentions)
Nicardipine hydrochloride, by Merck Group (1 mentions)
Flavopiridol hydrochloride hydrate, by Merck Group (1 mentions)
Vinblastine sulfate salt, by Merck Group (1 mentions)
Verapamil hydrochloride, by Merck Group (1 mentions)
Calcein acetoxymethyl ester calcein am, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Concanamycin a, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Doxorubicin hydrochloride, by Merck Group (1 mentions)
Dmem medium, by Lonza (1 mentions)
5 protocols using mitoxantrone dihydrochloride
1
Western Blotting Antibodies and Reagents
2
Cell Line Treatment and Analysis
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For short-term exposure, cell lines were plated in 6-well plates at a confluency of 70%. Cell lines were treated with 100 nmol/L of mitoxantrone dihydrochloride (Sigma-Aldrich), 100 nmol/L of daunorubicin dihydrochloride (Sigma-Aldrich), or vehicle (DMSO 0.1% v/v). Cell lysates or cell pellets were harvested after 48 hours of treatment for analysis by western blot and IHC.
3
Phytochemicals and Mitoxantrone Evaluation
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The phytochemicals malvidin chloride (CAS no. 643-84-5), pelargonidin chloride (CAS no. 134-04-3; purity ≥95%) and chemotherapeutic drug mitoxantrone dihydrochloride (CAS no. 90476-82-3; purity ≥97%) were obtained from Sigma-Aldrich, Germany. All other chemicals used in the study were of analytical grade.
4
Mitoxantrone-based Liposome Characterization
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Mitoxantrone dihydrochloride, NaTC, NaTDC and the spin probes 5-DSA, 12-DSA and 16-DSA were analytical grade and supplied by Sigma Aldrich (St. Louis, MO, USA). All the compounds were used without further purification. Experiments were performed in 0.1 M phosphate buffer (pH 7.4) and 0.1 M carbonate buffer (pH 10) and deionized water (18.2 MΩcm, Mili-Q water purification system) was used for the preparation of solutions.
5
Cytotoxicity Assay Protocol
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Trypsin (Trypsin-EDTA solution), adenosine triphosphate (ATP), propidium iodide, vinblastine sulfate salt, mitoxantrone dihydrochloride, flavopiridol hydrochloride hydrate, doxorubicin hydrochloride, verapamil hydrochloride, nicardipine hydrochloride, fumitremorgin C (FTC) bortezomib, concanamycin A and calcein-acetoxy methyl ester (calcein-AM) were purchased from Sigma-Aldrich (Castle Hill, NSW). penicillin-streptomycin solution (10000 U/ml penicillin 10000 µg/ml streptomycin) and MTT (3-(4,5-dimethylthiazol-2-yl-)-2,5-diphenyltetrazolium bromide) were purchased from ThermoFisher Scientific (Scoresby, Vic). DMEM medium was purchased from Lonza (Vic, Australia) and foetal bovine serum (FBS) from Biological Bovogen Ltd (Vic, Australia).
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