Red blood cell lysis

Manufactured by Lonza

Sourced in United Kingdom

The red blood cell lysis product is a laboratory tool designed to disrupt the membranes of red blood cells, allowing the release of their contents. This facilitates the isolation and analysis of other cellular components, such as DNA or proteins, from the sample. The product functions by utilizing a specialized lysis buffer to selectively break down red blood cell membranes while preserving the integrity of other cell types.

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Lab products found in correlation

Ter119 fitc, by BioLegend (1 mentions) Cd23 apc, by BioLegend (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hepes, by Merck Group (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions)

2 protocols using red blood cell lysis

Splenic B Cell and Bone Marrow Analysis

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To analyze splenic B cells, total splenocytes were obtained by mashing spleens on 70-µm filters followed by red blood cell lysis (Lonza). To analysis red blood cell content in the bone marrow, total bone marrow cells were flushed out of femora and tibiae using PBS. Single-cell suspensions were stained in the presence of Fc receptor-blocking antibodies (mouse CD16/32, clone 93) using the following antibodies (BioLegend): CD19-FITC (clone 1D3/CD19, 152403), B220-PerCP (clone RA3-6B2, 103233), CD93-PE (clone AA4.1, 136503), CD23-APC (clone B3B4, 101619), CD21-Pacific Blue (clone 7E9, 123413), Ter119-FITC (clone TER-119, 116205) and CD45-APC-Cy7 (clone 30-F11, 103115). Cell viability was measured using Zombie Yellow Fixable Viability kit (423103) or DAPI. Flow cytometry data were acquired on the NovoCyte flow cytometer (Acea Biosciences/Agilent Technologies) using NovoExpress (version 1.3.0) and analyzed using FlowJo (BD).

Takahama M., Patil A., Richey G., Cipurko D., Johnson K., Carbonetto P., Plaster M., Pandey S., Cheronis K., Ueda T., Gruenbaum A., Kawamoto T., Stephens M, & Chevrier N. (2024). A pairwise cytokine code explains the organism-wide response to sepsis. Nature Immunology, 25(2), 226-239.

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Isolation of Immune Cell Subsets

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Blood, bone marrow, spleen, and sub-mandibular lymph nodes (SMLNs) were collected in RPMI 1640 medium (Sigma) supplemented with HEPES (1M; Sigma), Penicillin–Streptomycin (Sigma), and 3% fetal bovine serum (FBS) (Life Technologies). Spleens and SMLNs were mashed through a 70 µm filter (Fisherbrand, UK) to yield a single cell suspension, followed by red blood cell lysis (Lonza, UK). Blood was subjected to two red blood cell lyses. Bones were opened at the knee joint and centrifuged to collect the marrow, which subsequently underwent red blood cell lysis.

O'Boyle C., Haley M.J., Lemarchand E., Smith C.J., Allan S.M., Konkel J.E, & Lawrence C.B. (2019). Ligature-induced periodontitis induces systemic inflammation but does not alter acute outcome after stroke in mice. International Journal of Stroke, 15(2), 175-187.

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