Anti tbx20

Manufactured by Merck Group

Anti-TBX20 is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the TBX20 protein, which plays a role in cardiac development. This product can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of TBX20 in biological samples.

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Lab products found in correlation

Ripa lysis buffer, by Beyotime (1 mentions) Ecl kit, by Merck Group (1 mentions) Anti calponin, by Abcam (1 mentions) Anti ve cadherin, by R&D Systems (1 mentions) Anti α sma, by Abcam (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Anti sm22α, by Abcam (1 mentions) Anti smad2, by Huabio (1 mentions) Anti smad3, by Huabio (1 mentions) Anti cd31, by Huabio (1 mentions) Facsaria 2 machine, by BD (1 mentions) Lipidtox, by Thermo Fisher Scientific (1 mentions)

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TBX20 (2 citations)

2 protocols using anti tbx20

Protein Expression Analysis of ASCs

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Protein was extracted from ASCs using RIPA lysis buffer (Beyotime) and then was quantified using a BCA Protein Assay Kit (Thermo Fisher). Ten to fifty microgram of each protein sample was separated via SDS-PAGE and electro-transferred onto PVDF membranes. Following blockade with PBST containing 5% BSA, the membranes were incubated with the following primary antibodies overnight at 4°C: anti-β-Actin (Santa Cruz); anti-TBX20 (sigma); anti-α-SMA (abcam); anti-Calponin (abcam); anti-SM22α (abcam); anti-Smad2 (HuaBio); anti-Smad3 (HuaBio); anti-phospho-Smad2 (HuaBio); anti-phospho-Smad3 (HuaBio); anti-CD31 (HuaBio); anti-VE-cadherin (R&D); and anti-VEGFR1 (HuaBio). The membranes were then washed four times with Tris-buffered saline/0.5% Tween-20 and incubated at 37°C for 1 h with the secondary antibodies (1:3,000; Thermo Fisher). Antigen and antibody complexes were detected by using an ECL Kit (Millipore). Immunoblots were quantified using Image Lab (version 6.0) software.

Ji Y., Ma Y., Shen J., Ni H., Lu Y., Zhang Y., Ma H., Liu C., Zhao Y., Ding S., Xiang M, & Xie Y. (2021). TBX20 Contributes to Balancing the Differentiation of Perivascular Adipose-Derived Stem Cells to Vascular Lineages and Neointimal Hyperplasia. Frontiers in Cell and Developmental Biology, 9, 662704.

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Epicardial Adipocyte Immunophenotyping by Flow Cytometry

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Dissociated epicardial adipocytes were fixed with 4% PFA for 45 min at room temperature, adipocytes were then washed with PBS buffer containing 3% BSA and 0.1% (V/V) Triton X-100 (PBST). Cells were blocked 10 min with 700 mL in blocking buffer (3% normal goat serum, 2% BSA and 0.1% (V/V) Triton X-100 in PBS) under gentle agitation, followed by primary antibody incubation for 1.5 h at room temperature with continuous agitation in blocking buffer. Subsequently, adipocytes were incubated with Alexa fluor-conjugated secondary antibodies: anti-mouse Alexa 647 (1:600, Cell Signaling, 4410S), anti-rabbit Alexa 555 (1:600, Cell Signaling, 4413s), 1:250 lipidTOX (Thermo Fisher, H34475) and DAPI (1 mg/mL) for 1 h at room temperature. Finally, cells were washed prior flow cytometry analysis with FACSAriaII machine (BD Biosciences). Analysis was performed using FlowJo 10.6.2 software.
The primary antibodies used for flow cytometry analysis were anti-Tbx20 (1:150, Sigma, sab1409545), anti-cardiac troponin t (1:250, GeneTex, GTX10214) and anti-HOXC8 (1:200, Thermo Fisher Scientific, PA5-41629).

Morocho-Jaramillo P.A., Kotlar-Goldaper I., Zakarauskas-Seth B.I., Purfürst B., Filosa A, & Sawamiphak S. (2024). The zebrafish heart harbors a thermogenic beige fat depot analog of human epicardial adipose tissue. Cell reports, 43(3).

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