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L methionine

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L-methionine is a type of amino acid that is commonly used in laboratory equipment. It serves as a core component in various processes and applications within the scientific field. The detailed function and intended use of L-methionine in lab equipment requires further information to present in an unbiased and factual manner.

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Lab products found in correlation

L alanine, by Fujifilm (6 mentions) L valine, by Fujifilm (6 mentions) L serine, by Fujifilm (5 mentions) L isoleucine, by Fujifilm (5 mentions) L phenylalanine, by Fujifilm (5 mentions) L threonine, by Fujifilm (5 mentions) L leucine, by Fujifilm (4 mentions) L glutamine, by Fujifilm (3 mentions) L aspartic acid, by Fujifilm (3 mentions) L glutamic acid, by Fujifilm (3 mentions) L glycine, by Fujifilm (3 mentions) L proline, by Fujifilm (3 mentions) L tryptophan, by Fujifilm (3 mentions) L tyrosine, by Fujifilm (3 mentions) L histidine, by Fujifilm (3 mentions) L asparagine monohydrate, by Fujifilm (2 mentions) L arginine, by Fujifilm (2 mentions) L asparagine, by Fujifilm (2 mentions) L glutamate, by Fujifilm (2 mentions) L lysine, by Fujifilm (2 mentions)

Close spelling variants of this product

L-methionine sulfone (7 citations) Methionine (3 citations)

9 protocols using l methionine

1

Biochemical Compounds Procurement Protocol

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NAD disodium salt, d-(–)-3-phosphoglyceric acid disodium salt, and l-homocysteine were purchased from Sigma-Aldrich Co., Ltd. (St. Louis, MO, USA). l-Alanine, l-valine, l-methionine, l-homoserine, l-serine, and N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), DTT, NADH, and isopropyl-β-d-thiogalactopyranoside (IPTG) were purchased from FUJIFILM Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Okamura E., Ohtaka K., Nishihama R., Uchida K., Kuwahara A., Mochida K, & Hirai M.Y. (2021). Diversified amino acid-mediated allosteric regulation of phosphoglycerate dehydrogenase for serine biosynthesis in land plants. Biochemical Journal, 478(12), 2217-2232.
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2

Taste Receptor Activation by Amino Acids

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Agar (purified powder) was obtained from Sigma-Aldrich (St Louis, MO, USA). As tastants, D-fructose (CAS: 57-48-7, Wako Pure Chemical Industries, Ltd., Osaka, Japan), as well as L-alanine (ALA; CAS: 56-41-7), L-arginine monohydrochloride (ARG; CAS: 1119-34-2), L-asparagine monohydrate (ASN; CAS: 5794-13-8), L-aspartic acid (ASP; CAS: 56-84-8), L-cysteine monohydrochloride hydrate (CYS; CAS: 7048-04-6), L-histidine monohydrochloride (HIS; CAS: 645-35-2), L-isoleucine (ILE; CAS: 73-32-5), L-leucine (LEU; CAS: 61-90-5), L-lysine monohydrochloride (LYS; CAS: 657-27-2), L-glutamine (GLN; CAS: 56-85-9), L-glutamic acid (GLU; CAS: 56-86-0), L-glycine (GLY; CAS: 56-40-6), L-methionine (MET; CAS: 63-68-3), L-phenylalanine (PHE; CAS: 63-91-2), L-proline (PRO; CAS: 147-85-3), L-serine (SER; CAS: 56-45-1), L-threonine (THR; CAS: 72-19-5), L-tryptophan (TRP; CAS: 73-22-3), L-tyrosine (TYR; CAS: 60-18-4) and L-valine (VAL; CAS: 72-18-4) were used at concentrations of 10 mmol l−1 (TYR was used in 0.5 mmol l−1 due to low solubility). Amino acids were obtained from Wako Pure Chemical Industries, Ltd., Osaka, Japan (TYR, SER, CYS, VAL), Sigma-Aldrich, St Louis, MO, USA (ILE, PRO) or Nacalai Tesque, Kyoto, Japan (ALA, ARG, ASN, ASP, LEU, LYS, GLN, GLY, MET, PHE, THR, TRP, TYR).
Kudow N., Miura D., Schleyer M., Toshima N., Gerber B, & Tanimura T. (2017). Preference for and learning of amino acids in larval Drosophila. Biology Open, 6(3), 365-369.
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3

Synthesis and Analysis of Amino Acids

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L-Kynurenine sulfate salt, KYNA, and L-tyrosine disodium salt hydrate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). L-Alanine, L-arginine, L-asparagine, L-aspartate, L-cysteine hydrochloride monohydrate, L-glutamine, L-glutamate, L-glycine, L-histidine, L-isoleucine, L-leucine hydrochloride, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, and L-valine were purchased from Wako Pure Chemical Industries (Osaka, Japan). Rodent diet (MF) was obtained from Oriental Yeast Co., Ltd (Tokyo, Japan). All other chemicals were of the highest commercially available purity.
Sekine A., Okamoto M., Kanatani Y., Sano M., Shibata K, & Fukuwatari T. (2015). Amino acids inhibit kynurenic acid formation via suppression of kynurenine uptake or kynurenic acid synthesis in rat brain in vitro. SpringerPlus, 4, 48.
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4

Evaluation of Vitamin B1-Deficient Diet

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Vitamin-free milk casein and gelatinized cornstarch, mineral mixture (AIN-93-G-MX),15 vitamin mixture (AIN-93-MX),15 vitamin B1–free vitamin mixture, dextrin, and cellulose were obtained from Oriental Yeast Co., Ltd. (Tokyo, Japan) l-Methionine was obtained from Wako Pure Chemical Industries Ltd. (Osaka, Japan) Corn oil was obtained from Ajinomoto Co., Inc. (Tokyo, Japan) The composition of the 2 diets, a nutritionally complete diet (referred to as control diet) and a vitamin B1–free diet, is shown in Table 1.
Tsuji A., Nakamura T, & Shibata K. (2017). Effects of Mild and Severe Vitamin B1 Deficiencies on the Meiotic Maturation of Mice Oocytes. Nutrition and Metabolic Insights, 10, 1178638817693824.
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5

Amino Acid and Metal Complex Preparation

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l-Alanine, l-proline, l-serine, l-threonine, l-aspartic acid, l-glutamic acid, l-methionine, citric acid, [RuCl2(p-cymene)]2 (1) and deuterium oxide (D2O) were purchased from Wako Pure Chemical Industries (Osaka, Japan). l-Cysteine, l-penicillamine and sodium 3-(trimethylsilyl)-1-propanesulfonate (DSS) was obtained from Sigma-Aldrich (St. Louis, USA). S-Methyl-l-cysteine was purchased from Tokyo Chemical Industry (Tokyo, Japan). l-Valine was obtained from Kanto Chemical Company (Tokyo, Japan). These reagents were used without further purification.
Aizawa S.I., Takizawa K, & Aitani M. (2019). Mechanistic study on substitution reaction of a citrato(p-cymene)Ru(ii) complex with sulfur-containing amino acids. RSC Advances, 9(43), 25177-25183.
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6

Analytical-Grade Chemical Compounds

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Propionic acid (PA), butyric acid (BA), L-glycine (Gly), L-alanine (Ala), L-serine (Ser), L-threonine (Thr), L-cysteine (Cys), L-methionine (Met), L-valine (Val), L-leucine (Leu), L-isoleucine (Ile), L-phenylalanine (Phe), L-tryptophan (Trp), L-aspartic acid (Asp), L-glutamic acid (Glu), L-asparagine (Asn), L-glutamine (Glu), L-histidine (His), L-asparagine monohydrate (Arg), L-tyrosine (Tyr), and L-ascorbic acid were purchased from FUJIFILM Wako (Osaka, Japan). Acetic acid (AA), L-lysine (Lys), and sodium butyrate were purchased from Nacalai Tesque (Kyoto, Japan), and proline (Pro) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All chemicals were of analytical grade.
Takakuwa A., Nakamura K., Kikuchi M., Sugimoto R., Ohira S., Yokoi Y, & Ayabe T. (2019). Butyric Acid and Leucine Induce α-Defensin Secretion from Small Intestinal Paneth Cells. Nutrients, 11(11), 2817.
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7

Formulation and Characterization of VD3

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Materials used in this study were purchased from the following commercial vendors: VD3 from Cayman Chemical (Ann Arbor, MI, USA). Excipients (special grade)—L-alanine (Ala), L-methionine (Met), L-phenylalanine (Phe), L-leucine (Leu), L-valine (Val) and L-isoleucine (Ile)—and reagents—ethanol (99.5%) (reagent grade), acetonitrile (HPLC grade) and polyoxyethylene (20) sorbitan monolaurate (biochemistry grade)—from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). 2 mL VIST glass vials obtained from Daiwa Special Glass (Osaka, Japan) and rubber stoppers (F5-43) obtained from Sumitomo Rubber Industries (Hyogo, Japan) were used as the packing materials.
Miyamoto K., Yanagisawa M., Taga H., Yamaji H., Akita T, & Yamashita C. (2021). Optimization of Very Low-Dose Formulation of Vitamin D3 with Lyophilizate for Dry Powder Inhalation System by Simple Method Based on Time-of-Flight Theory. Pharmaceutics, 13(5), 632.
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8

Amino Acid Reagent Preparation

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MEM essential amino acids solution, MEM non-essential amino acids solution, l-cystine, l-arginine, l-histidine, l-Isoleucine, l-leucine, l-lysine, l-methionine, l-phenylalanine, l-threonine, l-tryptophan, l-tyrosine, l-valine, William's Medium E (WME), Dulbecco's modified Eagle's medium (DMEM), and hanks' balanced salt solution (HBSS), were purchased from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Proteinase inhibitor cocktail was purchased from Nacalai Tesque, (Kyoto, Japan). Na2S4 was purchased from Dojindo Molecular Technologies, Inc (Kumamoto, Japan). β-(4-hydroxyphenyl)ethyl iodoacetamide (HPE-IAM) was purchased from Molecular Biosciences (CO, USA). All other reagents and chemicals were of the highest grades available.
Akiyama M., Unoki T., Aoki H., Nishimura A., Shinkai Y., Warabi E., Nishiyama K., Furumoto Y., Anzai N., Akaike T., Nishida M, & Kumagai Y. (2022). Cystine-dependent antiporters buffer against excess intracellular reactive sulfur species-induced stress. Redox Biology, 57, 102514.
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9

Purified HA and CS Extraction

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Purified HA from chicken combs and CS from shark cartilage were obtained from Seikgaku Corp. (Tokyo, Japan). D-sorbitol, glycine, L-glutamate, monosodium L-glutamate, L-methionine, D-glucose, maltose monohydrate, xylitol, and D-α-tocopherol were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan).
Nogami E., Watanabe I., Hoshi H., Kasahara M., Honda N., Sato M, & Suzuki K. (2019). D-sorbitol can keep the viscosity of dispersive ophthalmic viscosurgical device at room temperature for long term. Scientific Reports, 9, 16815.
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