Total RNA was extracted and purified from CAOV3 and OVCAR3 cells using a Trizol™ Plus RNA Purification Kit (Invitrogen) according to the manufacturer' protocol. Reverse transcription was conducted using a High-Capacity RNA-to-cDNA Kit (Invitrogen) according to the manufacturer's protocol. qRT-PCR was conducted on an ABI PRISM 7300 PCR sequence detection system (Applied Biosystems, Foster City, CA, USA) according to the manufacturer's protocol. The expression of lncRNA XIST was probed using a TaqMan non-coding RNA assay (Applied Biosystems). The expression of hsa-miR-214-3p was probed using a TaqMan advanced miRNA Assay (Applied Biosystems). Relative gene expression levels were calculated as fold changes using the (2−ΔΔCt) method.
Abi prism 7300 pcr sequence detection system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ABI PRISM 7300-PCR sequence detection system is a real-time PCR instrument designed for gene expression analysis and other quantitative PCR applications. The system uses fluorescence detection to monitor the amplification of target DNA sequences in real-time.
Automatically generated - may contain errors
Lab products found in correlation
Kingfisher dna extraction system, by Thermo Fisher Scientific (3 mentions)
Invimag stool dna kit, by Stratec (3 mentions)
Taqman advanced mirna assay, by Thermo Fisher Scientific (1 mentions)
Taqman non coding rna assay, by Thermo Fisher Scientific (1 mentions)
Trizol plus rna purification kit, by Thermo Fisher Scientific (1 mentions)
High capacity rna to cdna kit, by Thermo Fisher Scientific (1 mentions)
5 protocols using abi prism 7300 pcr sequence detection system
1
Quantifying lncRNA XIST and miR-214-3p
2
Multiplex qPCR for Gut Pathogens
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Real-time detection was performed on DNA samples targeting the following set of pathogens that were detected by 16S profiling: Clostridium difficile, Clostridium perfringens, Haemophilus parainfluenzae, Klebsiella pneumonia and Streptococcus pneumonia. The target organisms, their respective genetic loci targeted by qPCR and the primer (and probe) sequences used are listed in Supplementary Table 2 . Specific PCR conditions employed were adjusted per target locus and was based on the PCR protocols provided in the respective literature references from which the primer (and probe) sequences were obtained (Supplementary Table 2 ). Amplification and detection were performed using the ABIPRISM 7300-PCR sequence detection system (Applied Biosystems. Foster City, CA), detecting the fluorescent products in the last step of each amplification cycle. After amplification, melting curve analysis was employed to establish PCR-product specificity in case of SYBR Green based detection. Positive and negative controls (other bacterial species) were included in each qPCR run. Gene copy numbers per gram of faeces were extrapolated for each sample, using positive-control template DNA and standard curves generated in triplicate and linear Ct-value regression in serial 10-fold template dilutions.
3
Fecal DNA Extraction and Quantitative PCR
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Deoxyribonucleic acid (DNA) was isolated from faecal samples that were stored at −80°C until analysed. Samples were pre‐treated and DNA was extracted using an automated KingFisher DNA extraction system (Thermo Fisher Scientific Oy, Vantaa, Finland) and InviMag Stool DNA kit (Stratec Molecular, Berlin, Germany), as previously described 11 . The standard DNA for quantitative polymerase chain reaction was also prepared as previously described 11 . All DNA samples were stored at −20°C until analysed. Quantitative PCRs were completed as previously described 12 , 13 . PCR amplification and detection were performed with an ABI PRISM 7300‐PCR sequence detection system (Applied Biosystems, Foster City, CA, USA). The primers used in the analyses are described in Table S1 .
4
Automated DNA Extraction from Fecal Samples
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Fecal samples were stored at -80 °C until analyzed. They were pretreated and DNA was extracted using an automated KingFisher DNA extraction system (Thermo Fisher Scientific Oy, Vantaa, Finland) and InviMag Stool DNA kit (Stratec Molecular, Berlin, Germany), as previously described (33) . The standard DNA for quantitative PCR (qPCR) was prepared as described elsewhere (33) . All DNA samples were stored at -20 °C until analyzed. Quantitative PCRs were conducted as previously described (34, 35) . PCR amplification and detection were performed with an ABI PRISM 7300-PCR sequence detection system (Applied Biosystems, Foster City, CA).
5
Automated Stool DNA Extraction and qPCR
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Samples were pretreated and DNA extracted using the InviMag Stool DNA kit (Stratec Molecular, Berlin, Germany) and the automated KingFisher DNA extraction system (Thermo Fisher Scientific Oy, Vantaa, Finland), as previously described (30) . DNA samples were stored at -20 °C until analyzed. Quantitative PCRs were conducted as described elsewhere (31, 32) . For quantitative PCR, the standard DNA was prepared as previously (30) . Detection and PCR amplification were performed with an ABI PRISM 7300-PCR sequence detection system (Applied Biosystems, Foster City, CA).
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