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Multivision polymer detection system anti rabbit hrp and anti mouse ap polymers

Manufactured by Thermo Fisher Scientific
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The MultiVision polymer detection system is a lab equipment product that utilizes anti-rabbit/HRP and anti-mouse/AP polymers for polymer-based detection. It is designed to provide a reliable and efficient method for detecting target proteins or antigens in various biological samples.

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Lab products found in correlation

Ultra 5 block, by Thermo Fisher Scientific (2 mentions) 24 pax 5, by Abcam (2 mentions)

2 protocols using multivision polymer detection system anti rabbit hrp and anti mouse ap polymers

1

Parotid Gland Tissue Analysis of pSS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed (4%), paraffin-embedded parotid gland tissue sections (4 μm) of five pSS patients from the inception cohort were included. Patients were selected based on the presence of LELs (identified by H&E staining). After deparaffinization and heat-induced antigen retrieval in sodium citrate buffer (pH 6.0) for 20 min and endogenous peroxidase (0.3% H2O2) blocking, Ultra V Block (ThermoFisher Scientific) was applied for 5 min. Slides were incubated overnight at 4 °C with 2.5 μg/mL monoclonal mouse anti-human PAX5 antibody (BD Biosciences, clone 24/Pax-5) and 1 μg/mL monoclonal rabbit antihuman CD11c antibody (Abcam, clone EP1347Y) diluted in PBS/1% BSA/0.05% Tween-20. Secondary antibody-based detection was performed using the MultiVision polymer detection system (anti-rabbit/HRP and anti-mouse/AP polymers; ThermoFisher Scientific) according to the manufacturer’s instructions.
Verstappen G.M., Ice J.A., Bootsma H., Pringle S., Haacke E.A., de Lange K., van der Vries G.B., Hickey P., Vissink A., Spijkervet F.K., Lessard C.J, & Kroese F.G. (2020). Gene expression profiling of epithelium-associated FcRL4+ B cells in primary Sjögren’s syndrome reveals a pathogenic signature. Journal of autoimmunity, 109, 102439.
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2

Parotid Gland Tissue Analysis of pSS

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed (4%), paraffin-embedded parotid gland tissue sections (4 μm) of five pSS patients from the inception cohort were included. Patients were selected based on the presence of LELs (identified by H&E staining). After deparaffinization and heat-induced antigen retrieval in sodium citrate buffer (pH 6.0) for 20 min and endogenous peroxidase (0.3% H2O2) blocking, Ultra V Block (ThermoFisher Scientific) was applied for 5 min. Slides were incubated overnight at 4 °C with 2.5 μg/mL monoclonal mouse anti-human PAX5 antibody (BD Biosciences, clone 24/Pax-5) and 1 μg/mL monoclonal rabbit antihuman CD11c antibody (Abcam, clone EP1347Y) diluted in PBS/1% BSA/0.05% Tween-20. Secondary antibody-based detection was performed using the MultiVision polymer detection system (anti-rabbit/HRP and anti-mouse/AP polymers; ThermoFisher Scientific) according to the manufacturer’s instructions.
Verstappen G.M., Ice J.A., Bootsma H., Pringle S., Haacke E.A., de Lange K., van der Vries G.B., Hickey P., Vissink A., Spijkervet F.K., Lessard C.J, & Kroese F.G. (2020). Gene expression profiling of epithelium-associated FcRL4+ B cells in primary Sjögren’s syndrome reveals a pathogenic signature. Journal of autoimmunity, 109, 102439.
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