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Agarose

Manufactured by Sinopharm
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Sourced in China

Agarose is a polysaccharide derived from red seaweed. It is commonly used as a matrix material in gel electrophoresis for the separation and purification of biomolecules, such as DNA, RNA, and proteins.

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Lab products found in correlation

E coli bl21 de3, by Tiangen Biotech (3 mentions) Plasmid pet 30a, by Tiangen Biotech (3 mentions) Carrageen, by Sinopharm (2 mentions) Sodium cellulose, by Sinopharm (2 mentions) Alginate, by Sinopharm (2 mentions) Soluble starch, by Sinopharm (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Natural graphite powder, by Sinopharm (1 mentions) Nanopure water systems uv, by Thomas Scientific (1 mentions) Lipofectamine 3000 lipo3000, by Thermo Fisher Scientific (1 mentions) Polyvinylidene difluoride pvdf membrane, by Merck Group (1 mentions) Sybr premix ex taq 2, by Takara Bio (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions) Paraformaldehyde (pfa), by Beyotime (1 mentions) Lysotracker green, by Beyotime (1 mentions) Fibrinogen, by Solarbio (1 mentions) Precision plus protein dual color standard, by Bio-Rad (1 mentions) Urokinase, by Maclin Biochemical Technology (1 mentions) Ir 780 iodide, by Merck Group (1 mentions) Cetyltrimethylammonium chloride, by J&K Scientific (1 mentions)

9 protocols using agarose

1

Graphite-Agarose Composite Synthesis

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Natural graphite powder, agarose, EB, and other chemicals were purchased from Sinopharm Chemical Reagent Corp. and used without further purification. Deionized water (16 MΩ cm) was obtained from a Nanopure Water Systems UV (Thomas Scientific, Swedesboro, NJ).
Li J., Yang Y., Mao Z., Huang W., Qiu T, & Wu Q. (2016). Enhanced Resolution of DNA Separation Using Agarose Gel Electrophoresis Doped with Graphene Oxide. Nanoscale Research Letters, 11, 404.
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2

Detailed Assembly of Tetracycline Aptamer Sensors

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All specific sequences for assembly of Tds are shown in Supporting Information Table S1. All the DNA and RNA sequences were synthesized by Takara Bio, Inc. (Dalian, China). DNA sequences were designed by computer program RNA STRUCTURE 5.7. Agarose (biochemical grade) was bought from Sinopharm Chemical Reagent Co., Ltd. Gel red, DNase I, and RNase A were obtained from the KeyGEN Institute of Biotechnology (Jiangsu, China). Lyso-Tracker Green, 4% paraformaldehyde, and DAPI were purchased from Beyotime Biotechnology. 5-Diphenyl-2-H-tetrazolium bromide (MTT, Sigma–Aldrich, USA) were used as received. Lipofectamine®3000 (lipo3000) was obtained from Invitrogen. Polyvinylidene difluoride (PVDF) membranes and the enhanced chemiluminescence (ECL) reagents were obtained from Millipore (USA). The primary antibodies used in this study were rabbit anti-EGFR (Abways, Shanghai, China) and rabbit anti-β-actin (Abways, Shanghai, China). Horseradish peroxidase-conjugated AffiniPure goat anti-rabbit IgG secondary antibody was bought from Abways Technology (Shanghai, China). SYBR® Premix Ex Taq™ II was purchased from Takara Bio, Inc. (Dalian, China). All of the other chemicals and reagents were analytic grade and used without further purification.
Han X., Xu X., Wu Z., Wu Z, & Qi X. (2021). Synchronous conjugation of i-motif DNA and therapeutic siRNA on the vertexes of tetrahedral DNA nanocages for efficient gene silence. Acta Pharmaceutica Sinica. B, 11(10), 3286-3296.
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3

Purification and Characterization of Urokinase

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Ammonium sulfate (NH4)2SO4), Tris (hydroxymethyl) aminomethane, agarose, and ethylene diamine tetraacetic acid (EDTA) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Urokinase was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Fibrinogen was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). Thrombin was purchased from Abmole (Houston, TX, USA). An Omni-EasyTM One-step PAGE Gel Fast Preparation Kit (12.5%, Catalog No. PG213) was purchased from Shanghai Epizyme Biomedical Technology Co., Ltd. (Shanghai, China). A 10× Tris/Glycine/SDS Buffer (Catalog 1610732), 4× Laemmli Sample Buffer (Catalog No. 1610747) and Precision Plus Protein Dual Color Standards with MW of 10–250 kDa (Catalog 1610374) were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). The bicinchoninic acid (BCA) protein quantitation kit (Catalog PA115-01) was purchased from Tiangen Biotech (Beijing, China) Co., Ltd. (Beijing, China). DEAE-Sepharose FF, Sephadex G-50, soybean trypsin inhibitor (SBTI), and phenyl methane sulfonyl fluoride (PMSF) were purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China). Epsilon amino caproic acid (EACA) was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). All reagents used were of analytical grade unless otherwise stated.
Jiang T., Zhang B., Zhang H., Wei M., Su Y., Song T., Ye S., Zhu Y, & Wu W. (2024). Purification and Properties of a Plasmin-like Marine Protease from Clamworm (Perinereis aibuhitensis). Marine Drugs, 22(2), 68.
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4

Biopolymer Extraction from Marine Macroalgae

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Plasmid pET-30a (+) and E. coli BL21 (DE3) were purchased from Tiangen Biotech Co., Ltd. (Beijing, China). Soluble starch, agarose, carrageen, sodium cellulose, and alginate were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, china). Grateloupia filicina, Chondrus ocellatus, and Scagassum were collected from Yangkou Beach in Qingdao, China.
Gu X., Fu L., Pan A., Gui Y., Zhang Q, & Li J. (2021). Multifunctional alkalophilic α-amylase with diverse raw seaweed degrading activities. AMB Express, 11, 139.
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5

Synthesis of Plasmonic Nanoparticles

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Chloroauric chloride (HAuCl4·4H2O), ethanol (≥99.7%), N,N‐dimethylformamide (DMF, ≥99%) and agarose were received from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Cetyltrimethylammonium chloride (CTAC, 99%) and sodium borohydride (NaBH4, 98%) were purchased from J&K Chemical Ltd. (Shanghai, China). Silver nitrate (AgNO3, 99.8%) and ascorbic acid (>99.0%) were obtained from Aladdin (China). 4‐Nitrobenzenethiol (4‐NBT) and IR‐780 iodide (IR780, 98%) were acquired from Sigma Aldrich (Shanghai, China). Thiolated PEG (M.W. = 5000) and carboxyl PEG thiol (M.W. = 5000) were purchased from Lingfeng Ltd. (Shanghai, China). Poly(vinylpolypyrrolidone) (PVP) was purchased from Hubei Gedian Human Wellbeing Pharmaceutical Co. (China). Nanopure water (18.2 MΩ) was used for all experiments. All materials were used as received without any further purification.
Wu Z., Deng B., Zhou Y., Xie H., Zhang Y., Lin L, & Ye J. (2023). Non‐Invasive Detection, Precise Localization, and Perioperative Navigation of In Vivo Deep Lesions Using Transmission Raman Spectroscopy. Advanced Science, 10(24), 2301721.
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6

Synthesis and Characterization of Nanomaterials

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The following chemicals were procured from Sigma Aldrich (MO, USA): silver nitrate (AgNO3), sodium borohydride (NaBH4), hydrogen tetrachloroaurat(III) (HAuCl4), L-ascorbic acid, and cetyltrimethylammonium bromide (CTAB). Additionally, the following chemicals were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China): Methyl methacrylate (MMA), Ammonium persulfate (APS), Palmitoyl oleyl Phosphatidylcholine (POPC), and Agarose. Phosphate Buffered Saline (PBS) and Fetal Bovine Serum (FBS) were procured from GIBCO Co., Ltd. (California, USA). Trypsin and high glycemic medium (Dulbecco’s modified Eagle’s medium, DMEM) were also acquired from GIBCO Co., Ltd. The CCK-8 kit was purchased from MCE (MedChemExpress); Perfluoropentane (PFP) and O-Carboxymethylchitosan (OCMC) were provided by Shanghai Aladdin Biochemical Technology Co., Ltd.
Tang X., Liu Y., Zhao M., He L., Guo J., Wang T., Li W, & Zhao J. (2024). Gold Nanorod-Loaded Nano-Contrast Agent with Composite Shell-Core Structure for Ultrasonic/Photothermal Imaging-Guided Therapy in Ischemic Muscle Disorders. International Journal of Nanomedicine, 19, 4121-4136.
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7

PDMS-based Photodynamic Therapy Protocol

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Polydimethylsiloxane (PDMS, Sylgard184) was purchased from Dow Corning Corp. (USA). PVP (Mw = 58 kDa) and sodium percarbonate (SPC) were bought from Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Sodium carbonate (SC), dimethyl sulfoxide (DMSO, AR), and agarose (BR) were provided by Sinopharm Chemical Reagent Co., Ltd (Beijing, China). Chlorins e6 (Ce6) was purchased from Frontier. F‐127 was purchased from Sigma (USA). 2,7‐Dichlorofluorescin diacetate (DCFH‐DA) was purchased from Beyotime Biotechnology Co., Ltd (China). Calcein‐AM/PI live/dead cell staining kit was provided by Yeasen Biotechnology Co., Ltd (Shanghai, China).
Liu P., Fu Y., Wei F., Ma T., Ren J., Xie Z., Wang S., Zhu J., Zhang L., Tao J, & Zhu J. (2022). Microneedle Patches with O2 Propellant for Deeply and Fast Delivering Photosensitizers: Towards Improved Photodynamic Therapy. Advanced Science, 9(25), 2202591.
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8

Recombinant Protein Expression in E. coli

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Plasmid pET-30a (+) and E. coli BL21 (DE3) were purchased from Tiangen (Beijing, China). Agarose was purchased from Sinopharm Group Chemical Reagent (Shanghai, China). His-tag-specific purification kits were purchased from GE Healthcare (Pittsburgh, PA, United States). Thin layer chromatography (TLC) silica gel plates were purchased from Kadel (Shanghai, China). Bio-Gel P-2 was purchased from Bio-Rad (Pittsburgh, PA, United States). RAW 264.7 macrophages were obtained from the Cell Bank, Chinese Academy of Sciences (Shanghai, China). LPS and L-monomethylarginine (L-NMMA) were purchased from Yuanye (Shanghai, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Fetal bovine serum (FBS) was obtained from Gibco (Grand Island, NY, United States). Enzyme Linked Immunoabsorbent Assay (ELISA) kits (TNF-α and IL-6) were purchased from AndHider (Qingdao, Shandong, China).
Gu X., Zhao L., Tan J., Zhang Q., Fu L, & Li J. (2022). Characterization of a novel β-agarase from Antarctic macroalgae-associated bacteria metagenomic library and anti-inflammatory activity of the enzymatic hydrolysates. Frontiers in Microbiology, 13, 972272.
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9

Extraction and Characterization of Marine Polysaccharides

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Plasmid pET-30a (+) and E. coli BL21 (DE3) were purchased from Tiangen Biotech Co., Ltd. (Beijing, China). Soluble starch, agarose, carrageen, sodium cellulose, and alginate were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, china). Grateloupia licina, Chondrus ocellatus, and Scagassum were collected from Yangkou Beach in Qingdao, China.
gu x., Fu L., Pan A., Gui Y., Zhang Q., & Li J. (2021). Multifunctional Alkalophilic α-Amylase With Diverse Raw Seaweed Degrading Activities.
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