Anti aldh1a1 antibody

Dulbecco's modified Eagle's medium (DMEM), phosphate buffered saline (PBS), penicillin/streptomycin, fetal bovine serum were purchased from Wisent (Montreal, Canada). Dithiothreitol (DTT) was purchased from Merck (Whitehouse Station, NJ). Iodoacetamide (IAA) was purchased from Sigma (St Louis, MO). Sequencing grade modified trypsin was purchased from Promega (Fitchburg, WI). The TMT labeling kit was purchased from Thermo-Pierce Biotechnology (Rockford, IL). Anti-VIM antibody, anti-CHMP2B antibody, anti-PDZK1 antibody, anti-VTI1A antibody, anti-Cdk1 antibody, anti-Cdc25C antibody, anti-Y15-p-Cdk1 antibody and anti-H3 antibody were from Proteintech (Wuhan, China). Anti-ALDH1A1 antibody, anti-β-actin antibody and anti-flag antibody were from Sigma (St Louis, MO). Anti-CD133 antibody was from Miltenyi Biotec (Bergisch Gladbach, Germany).

Anti-ZIP4 antibody (Proteintech) anti-HDAC4 (D15C3) antibody (Cell Signaling Technology), and anti-ALDH1A1 antibody (Sigma) were used as primary antibodies (1:100, 4 °C overnight). A VECTASTAIN^® Universal Quick HRP Kit (PK-7800) and a Vector NavaRED Substrate Kit Substrate Kit (SK-4800, Vector Laboratories, Burlingame, CA, USA) were used for detection. Tumor tissues from different groups of mice were examined in immunohistochemistry (IHC) staining. For IF Staining, Alexa Fluor antibodies (PE Donkey anti-Rabbit Cat.Log# 406421 and FITC Donkey anti-Goat Cat.Log# sc-3853) were used as secondary antibodies. Nuclei were counterstained using 4′, 6-diamidino-2-phenylindole (DAPI; Santa Cruz Biotechnology). The staining was analyzed using a Nikon Eclipse 80i microscope (Nikon, Badhoevedorp, The Netherlands).