α olig2

Manufactured by Merck Group

α-OLIG2 is a laboratory reagent used in research applications. It is a protein that plays a role in the development and differentiation of oligodendrocytes, a type of glial cell in the central nervous system. The core function of α-OLIG2 is to act as a transcription factor, regulating the expression of genes involved in oligodendrocyte lineage specification and maturation.

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Lab products found in correlation

Rabbit α ift88, by Proteintech (1 mentions) Mouse α γ tubulin, by Merck Group (1 mentions) Inpp5e, by Proteintech (1 mentions) α mouse alexafluor488, by Thermo Fisher Scientific (1 mentions) α rabbit alexafluor 488, by Thermo Fisher Scientific (1 mentions) Igg1 fitc, by Miltenyi Biotec (1 mentions) α phospho histone h3, by Cell Signaling Technology (1 mentions) Igg1 apc, by Miltenyi Biotec (1 mentions) α mouse alexa fluor 568, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Olig2

2 protocols using α olig2

Antibody Use for Cell Signaling Analysis

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The SMO antibody was raised in rabbits (Pocono Rabbit Farm and Laboratory Inc.) using antigens and procedures described in Rohatgi et. al. [43 (link)]; and used at a dilution of 1:500. The KIF7 antibody [41 (link)] was used at 1:1000. Monoclonal antibodies against NKX2.2 and ISL1 were used at 1:10 and obtained from the Developmental Studies Hybridoma Bank. Commercially available antibodies used were: rabbit α-FOXA2 (Millipore, 1:500) rabbit α-OLIG2 (1:200, Millipore), mouse α-acetylated α-Tubulin (1:5000, Sigma Aldrich), mouse α-γ-Tubulin (1:5000, Sigma Aldrich), rabbit α-IFT88 (1:500, Proteintech), rabbit α-IFT81 (1:200, Proteintech), INPP5E (1:200, Proteintech), GPR161 (1:100, Proteintech). The GLI2 [44 (link)] and ARL13B [45 (link)] antibodies were described previously.

Goetz S.C., Bangs F., Barrington C.L., Katsanis N, & Anderson K.V. (2017). The Meckel syndrome- associated protein MKS1 functionally interacts with components of the BBSome and IFT complexes to mediate ciliary trafficking and hedgehog signaling. PLoS ONE, 12(3), e0173399.

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Immunophenotyping of Cell Populations

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The antibodies used in this study were α-CD44 (Dako, M708201), α-CD44-FITC (Miltenyi-Biotec, 130-098-210), α-CD133-APC (Miltenyi-Biotech, 130-098-829), α-phospho-histone H3 (Cell signaling, 9701), IgG1-FITC (Miltenyi-Biotec, 130-098-847), IgG1-APC (Miltenyi-Biotech, 130-098- 846), α-Olig2 (Millipore, ab9610), α-Mouse Alexa Fluor 488 (ThermoFisher Scientific, A11029), α-Mouse Alexa Fluor 568 (ThermoFisher Scientific, A11004), α-Rabbit Alexa Fluor 488 (ThermoFisher Scientific, A11008) and α-Rabbit Alexa Fluor 568 (ThermoFisher Scientific, A11011).

Brown D.V., Filiz G., Daniel P.M., Hollande F., Dworkin S., Amiridis S., Kountouri N., Ng W., Morokoff A.P, & Mantamadiotis T. (2017). Expression of CD133 and CD44 in glioblastoma stem cells correlates with cell proliferation, phenotype stability and intra-tumor heterogeneity. PLoS ONE, 12(2), e0172791.

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