Eczr 400 mhz nmr spectrometer

All reagents were purchased from Merck (Sigma-Aldrich, St. Louis, MO, USA) and Alfa (Alfa-Aesar, Ward Hill, MA, USA). Reactions were performed using an Anton-Paar Monowave 50 microwave reactor (Graz, Austria). The melting points were determined on a Kofler hot-plate apparatus HMK (Franz Kustner Nacht KG, Dresden, Germany) and are uncorrected. All ¹H- and ¹³C-NMR spectra were recorded on a JEOL ECZR 400 MHz NMR spectrometer (400 MHz for ¹H and 100 MHz for ¹³C, Jeol, Tokyo, Japan) in dimethyl sulfoxide-d₆ (DMSO-d₆). ¹H and ¹³C chemical shifts (δ) are reported in ppm. High-resolution mass spectra were measured using a high-performance liquid chromatograph Dionex UltiMate^® 3000 (Thermo Scientific, West Palm Beach, FL, USA) coupled with an LTQ Orbitrap XL^TM Hybrid Ion Trap-Orbitrap Fourier Transform Mass Spectrometer (Thermo Scientific) equipped with a HESI II (heated electrospray ionization) source in the positive and negative mode.

All reagents were purchased from Sigma-Aldrich and Acros, respectively. Anhydrous dichloromethane was distilled from calcium hydride. TLC experiments were performed on alumina-backed silica gel 60 F254 plates (Merck, Darmstadt, Germany). The plates were illuminated under UV (254 nm). The melting points were determined on Böetius PHMK 05 (Franz Küstner Nachf). Infrared (IR) spectra were collected on a Smart MIRacle^TM ATR ZnSe for the Nicolet^TM Impact 410 FT-IR Spectrometer (Thermo Scientific). All ¹H, ¹⁹F and ¹³C spectra were specified using a JEOL ECZR 400 MHz NMR spectrometer (400 MHz for ¹H, 101 MHz for ¹³C and 376 MHz for ¹⁹F, JEOL) in CDCl₃. Chemical shifts (δ) are reported in ppm. High-resolution mass spectra were measured on LTQ Orbitrap XL^TMHybrid Ion Trap-Orbitrap Fourier Transform Mass Spectrometer (Thermo Scientific) with injection into HESI II in the positive mode.

All reagents were purchased from Merck (Sigma-Aldrich, St. Louis, MO, USA) and Acros Organics (Thermo Fisher Scientific, Geel, Belgium). The melting points were determined on a Kofler hot-plate apparatus HMK (Franz Kustner Nacht KG, Dresden, Germany) and are uncorrected. The measurement of optical rotations was carried on Automatic polarimeter AA-10 (Optical Activity, Ramsey, UK). The concentration of samples is given in 10 mg/mL. Infrared (IR) spectra were recorded on a Smart MIRacle™ ATR ZnSe for Nicolet™ Impact 410 Fourier-transform IR spectrometer (Thermo Scientific, West Palm Beach, FL, USA). The spectra were obtained by the accumulation of 256 scans with 2 cm⁻¹ resolution in the region of 4000–650 cm⁻¹. All ¹H- and ¹³C-NMR spectra were recorded on a JEOL ECZR 400 MHz NMR spectrometer (400 MHz for ¹H and 101 MHz for ¹³C, Jeol, Tokyo, Japan) in dimethyl sulfoxide-d₆ (DMSO-d₆). ¹H and ¹³C chemical shifts (δ) are reported in ppm. The signal of the residual solvent (DMSO-d₆) was used as a reference. High-resolution mass spectra were measured using a high-performance liquid chromatograph Dionex UltiMate^® 3000 (Thermo Scientific) coupled with an LTQ Orbitrap XL^TM Hybrid Ion Trap-Orbitrap Fourier Transform Mass Spectrometer (Thermo Scientific) equipped with a HESI II (heated electrospray ionization) source in the positive mode.