Blood was isolated from the tail vein of transgenic mice and serum was obtained by centrifugation. To select transgenic mice with liver toxicity due to the uPA transgene, alanine aminotransferase (ALT) activity was measured using the transaminase CII-test kit (WAKO Pure Chemical Industries, Ltd.). uPA and ALT activities in the selected transgenic mice were determined by ELISA (Mouse uPA Activity Assay, Molecular Innovations) and DRI-Chem 3500 (Fujifilm, Tokyo, Japan), respectively.
H-alb concentration in chimeric mouse blood was measured by immunonephelometry in a JEOL BM6050 autoanalyzer (JEOL, Tokyo, Japan) using LX Reagent Eiken Alb II (Eiken Chemical, Tokyo, Japan). Creatinine (CRE) and blood urea nitrogen (BUN) levels were measured by the urease glutamate dehydrogenase method and enzyme method using EKDIA XL ‘Eiken’ CREIII and EKDIA XL ‘Eiken’ UN (Eiken Chemical, Tokyo, Japan), respectively, with a JEOL BM6050 autoanalyzer (JEOL, Tokyo, Japan).
Tateno C., Kawase Y., Tobita Y., Hamamura S., Ohshita H., Yokomichi H., Sanada H., Kakuni M., Shiota A., Kojima Y., Ishida Y., Shitara H., Wada N.A., Tateishi H., Sudoh M., Nagatsuka S.I., Jishage K.I, & Kohara M. (2015). Generation of Novel Chimeric Mice with Humanized Livers by Using Hemizygous cDNA-uPA/SCID Mice. PLoS ONE, 10(11), e0142145.
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