Human ht 12 expression beadchip arrays

Blood samples were collected, transported and processed in accordance with strict standard operating procedures. Briefly, peripheral blood was collected in Tempus Blood RNA Tubes (Applied Biosystems), which contain a proprietary reagent to lyse blood cells upon collection and permit stable storage and transportation of total RNA and transported to a central processing site within 5 days [16 (link)]. RNA was purified at a central biorepository using proprietary buffer according to the manufacturer’s instructions using the Tempus Spin RNA Isolation Reagent Kit (Applied Biosystems). RNA samples were assayed for quality (A260:A280 and A260:A230 ratios >1.8; RIN >7.0; Agilent BioAnalyzer) and concentration prior to labeling and processing. Whole genome gene expression profiles were determined using Illumina Human HT-12 Expression BeadChip Arrays. Each array targets more than 47,000 probes designed to cover genes from NCBI RefSeq Release 38 as well as legacy UniGene genes.

Details of the microarray analysis were described before [12 (link)]. Human HT-12 Expression BeadChip arrays (Illumina, San Diego, CA) were used for microarray hybridization to examine the global gene expression of hESC, hiPSC, and starting populations (CD34⁺ progenitors and fibroblasts). The NIH Gene Expression Omnibus has issued the accession numbers GSE44425 (Figure 1, Figure 5(c)) for the deposited microarray data related to the above manuscript.