Log In Sign up
The largest database of trusted experimental protocols

Anti nkr p1a

Manufactured by BD
Visit Supplier
Sourced in United States

Anti-NKR-P1A is a laboratory reagent used in flow cytometry applications. It is an antibody that specifically binds to the NKR-P1A cell surface receptor, which is expressed on natural killer cells. The primary function of this product is to facilitate the identification and analysis of natural killer cells in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Gallios cytometer, by Beckman Coulter (4 mentions) Anti tcrγδ, by BD (4 mentions) Anti tcrαβ, by BD (4 mentions) Anti cd4, by BD (4 mentions) Anti cd8α, by BD (4 mentions) Anti cd8β, by BD (4 mentions) Anti cd45ra, by BD (3 mentions) Anti cd103, by BioLegend (3 mentions) Anti cd62l, by BioLegend (3 mentions) Anti tlr4, by Novus Biologicals (2 mentions) Anti foxp3, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-NKR-P1A (10/78), (2 citations)

4 protocols using anti nkr p1a

1

Multiparametric Flow Cytometry Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, lymphocytes (2 × 105) from spleens and MLNs were labeled with mouse anti-rat monoclonal antibodies (mAb) conjugated to FITC, phycoerythrin (PE), peridinin-chlorophyll-a protein (PercP), allophycocyanin (APC), or BD Horizon™ BV421, as in previous studies [21 (link),22 (link)]. In this case, the mAb used were anti-CD4, anti-CD8α, anti-CD8β, anti-TCRαβ, anti-NKR-P1A, anti-TCRγδ, and anti-CD45RA (BD Biosciences, San Diego, USA). After staining with standard procedures [21 (link)], analyses were performed using a GalliosTM Cytometer (Beckman Coulter, Miami, FL, USA) at the CCiT-UB. All results were assessed by the FlowJo v.10 software.
Grases-Pintó B., Torres-Castro P., Marín-Morote L., Abril-Gil M., Castell M., Rodríguez-Lagunas M.J., Pérez-Cano F.J, & Franch À. (2019). Leptin and EGF Supplementation Enhance the Immune System Maturation in Preterm Suckling Rats. Nutrients, 11(10), 2380.
+ Open protocol
+ Expand
2

Multicolor Flow Cytometry of Gut Lymphocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometric analysis, 5x105 PPL, IEL and LPL were labelled with mouse anti-rat monoclonal antibodies conjugated with fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridininchlorophylla protein (PerCP) or allophycocyanin (APC). The antibodies used were anti-CD4, anti-CD8α, anti-CD8β, anti-TCRαβ, anti-TCRγδ, anti-NKR-P1A, anti-CD25 (BD Biosciences, Oxford, UK), anti-CD62L, anti-CD103 (Biolegend, San Diego, CA, USA) and anti-TLR4 (Novus Biologicals, Littleton, CO, USA). The cells were stained as previously described [19 (link)]. Briefly, lymphocytes were incubated with saturating amounts of antibodies in PBS-0.2% FBS-0.1% NaN3 (darkness, 20 min, 4 °C). Consecutively, the cells were washed, and fixed with 0.5% p-formaldehyde (darkness, until analysis, 4 °C). A negative control staining was included in each cell sample. Analyses were performed with a Gallios Cytometer (Beckman Coulter, Miami, FL, USA) in the Scientific and Technological Centres of the University of Barcelona (CCiTUB).
Camps-Bossacoma M., Pérez-Cano F.J., Franch À., Untersmayr E, & Castell M. (2017). Effect of a cocoa diet on the small intestine and gut-associated lymphoid tissue composition in an oral sensitization model in rats. The Journal of nutritional biochemistry, 42, 182-193.
+ Open protocol
+ Expand
3

Comprehensive Flow Cytometric Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometric analysis, splenocytes (2 × 105) were labelled with mouse anti-rat monoclonal antibodies (mAb) conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridininchlorophylla protein (PercP), allophycocyanin (APC) or APC-Cyanine (Cy)7, as in previous studies60 (link). In this case, the antibodies used were anti-CD4, anti-CD8α, anti-CD8β, anti-TCRαβ, anti-TCRγδ, anti-NKR-P1A, anti-CD25, anti-CD45RA (BD Biosciences, San Diego, USA), anti-CD62L, anti-CD103 (Biolegend, San Diego, CA, USA), anti-TLR-4 (Novus Biologicals, Littlon, CO, USA) and anti-Foxp3 (eBioscience). After staining with standard procedures20 (link), analyses were performed using a GalliosTM Cytometer (Beckman Coulter Inc., Miami, FL, USA) at the CCiT-UB. All results were assessed by the Flowjo v.10 software (TreeStar, Inc., Ashland, OR, USA).
Grases-Pintó B., Abril-Gil M., Castell M., Pérez-Cano F.J, & Franch À. (2019). Enhancement of immune maturation in suckling rats by leptin and adiponectin supplementation. Scientific Reports, 9, 1786.
+ Open protocol
+ Expand
4

Comprehensive Lymphocyte Subtyping by Flow Cytometry

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
IEL (2 × 105) were stained with anti-rat monoclonal antibodies (mAb) conjugated to fluorescein isothiocyanate (FITC), phycoerythrin (PE), peridininchlorophylla protein (PercP), allophycocyanin (APC) or APC-cyanine (Cy)7, as in previous studies (24 (link)). In this case, the mAb used were anti-CD4, anti-CD8α, anti-CD8β, anti-TCRαβ, anti-TCRγδ, anti-NKR-P1A, anti-CD25, anti-CD45RA (BD Biosciences, San Diego, USA), anti-CD62L, and anti-CD103 (Biolegend, San Diego, CA, USA). After standard procedures (20 (link)), samples were analyzed using a GalliosTM Cytometer (Beckman Coulter Inc., Miami, FL, USA) in the Scientific and Technological Centers of the University of Barcelona (CCiT-UB). The results were assessed by FlowJo version 10 software (TreeStar, Inc., Ashland, OR, USA). Lymphocyte subsets were defined as follows: Th (TCRαβ+ NKR-P1A− CD4+) cells; Tc TCRαβ+ (TCRαβ+ NKR-P1A− CD8+) cells; Tc TCRγδ+ (TCRγδ+ CD8+) cells; natural killer (NK) (NKR-P1A+ TCRαβ−) cells; NKT (NKR-P1A+ TCRαβ+) cells; CD4+ (CD4+ CD8−) cells; CD8+ (CD4− CD8+) cells; CD8αα+ (CD8α+ CD8β−); CD8αβ+ (CD8α+ CD8β+). Results are expressed as percentages of positive cells in the lymphocyte population selected according to their forward-scatter cells (FSC) and side-scatter characteristics (SSC) or in a particular selected population.
Grases-Pintó B., Abril-Gil M., Castell M., Rodríguez-Lagunas M.J., Burleigh S., Fåk Hållenius F., Prykhodko O., Pérez-Cano F.J, & Franch À. (2019). Influence of Leptin and Adiponectin Supplementation on Intraepithelial Lymphocyte and Microbiota Composition in Suckling Rats. Frontiers in Immunology, 10, 2369.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!