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Minispec nmr analyzer

Manufactured by Bruker
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Sourced in United States, Germany

The Minispec NMR analyzer is a compact and versatile nuclear magnetic resonance (NMR) spectrometer designed for a wide range of analytical applications. It utilizes NMR technology to provide non-destructive, rapid, and accurate measurements of various sample properties.

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Lab products found in correlation

D12492, by Research Diets (1 mentions) Oxymax clams, by Columbus Instruments (1 mentions) Mk 5000rq, by Muromachi Kikai (1 mentions) Accu chek compact plus device, by Roche (1 mentions) High fat diet (hfd), by Ssniff (1 mentions) Mcp 1, by R&D Systems (1 mentions)

15 protocols using minispec nmr analyzer

1

Body Composition and Telemetry in Mice

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Body composition (fat and muscle) of non-anesthetized mice was measured using the Bruker Minispec NMR analyzer (Bruker Optics). For activity and blood pressure measurements using telemetry, transmitters were implanted in 14 wk old mice and allowed to recover from the surgery for 2 wk. Blood pressure and physical activity were measured every 5 sec for 10 min (120 data points) at 3 a.m. (dark cycle) and 3 p.m. (light cycle), 3 d/wk for 5 wk.
Kang J.G., Sung H.J., Amar M.J., Pryor M., Remaley A.T., Allen M.D., Noguchi A.C., Springer D.A., Kwon J., Chen J., Park J.H., Wang P.Y, & Hwang P.M. (2016). Low ambient oxygen prevents atherosclerosis. Journal of molecular medicine (Berlin, Germany), 94(3), 277-286.
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2

High-Fat Diet and Exercise Effects on Metabolism

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For the high-fat diet study, mice were fed a rodent diet with 60 kcal% fat (cat. #D12492, Research Diets) for 8 wk. After the 8 wk period, the mice were switched to a normal diet with or without exercise training for an additional 4 wk during which time body measurements were made. The body composition of non-anesthetized mice was measured using the Minispec NMR analyzer (Bruker). While monitoring food intake and activity, metabolism was measured in the mice by indirect calorimetry (O2 consumption and CO2 production) using a Comprehensive Lab Animal Monitoring System (Oxymax/CLAMS, Columbus Instruments, OH).
Ma J., Wang P.Y., Zhuang J., Son A.Y., Karius A.K., Syed A.M., Nishi M., Wu Z., Mori M.P., Kim Y.C, & Hwang P.M. (2023). CHCHD4-TRIAP1 regulation of innate immune signaling mediates skeletal muscle adaptation to exercise. Cell reports, 43(1), 113626.
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3

Body Composition and Metabolism Analysis

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Body composition was assessed with the miniSpec NMR Analyzer (Bruker Optics, Billerica, MA, USA). For indirect caloric measurements, mice were individually housed in metabolic cages (LabMaster Home Cages System; TSE Systems, Chesterfield, MO, USA; or MK-5000RQ; Muromachi Kikai, Tokyo, Japan) for 2–3 d (20–22°C, 14 h light–10 h dark cycle). Mice were adapted to the metabolic cages for 2 d. Mice were provided with food and water ad libitum.
Hofer D.C., Zirkovits G., Pelzmann H.J., Huber K., Pessentheiner A.R., Xia W., Uno K., Miyazaki T., Kon K., Tsuneki H., Pendl T., Al Zoughbi W., Madreiter-Sokolowski C.T., Trausinger G., Abdellatif M., Schoiswohl G., Schreiber R., Eisenberg T., Magnes C., Sedej S., Eckhardt M., Sasahara M., Sasaoka T., Nitta A., Hoefler G., Graier W.F., Kratky D., Auwerx J, & Bogner-Strauss J.G. (2019). N-acetylaspartate availability is essential for juvenile survival on fat-free diet and determines metabolic health. The FASEB Journal, 33(12), 13808-13824.
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4

Body Composition Monitoring in Mice

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Whole body fat, fluids and lean muscle mass of isoflurane-anesthetized mice were determined biweekly using a Bruker Optics Minispec NMR analyzer (Billerica, MA) in accordance with the manufacturer's recommendations.
Blázquez-Medela A.M., Jumabay M., Rajbhandari P., Sallam T., Guo Y., Yao J., Vergnes L., Reue K., Zhang L., Yao Y., Fogelman A.M., Tontonoz P., Lusis A.J., Wu X, & Boström K.I. (2019). Noggin depletion in adipocytes promotes obesity in mice. Molecular Metabolism, 25, 50-63.
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5

Body Composition Measurement by NMR

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Body composition analysis was carried out as described previously [22 (link)]. Measurements of adipose and lean mass, expressed as percentage, were performed in a three-minute trial using the Minispec® NMR analyzer (mq 7.5; Bruker Optics, USA).
Tramullas M., Finger B.C., Dinan T.G, & Cryan J.F. (2016). Obesity Takes Its Toll on Visceral Pain: High-Fat Diet Induces Toll-Like Receptor 4-Dependent Visceral Hypersensitivity. PLoS ONE, 11(5), e0155367.
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6

Weekly Whole-Body Composition Analysis

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Whole body fat, fluids, and lean muscle mass of mice were determined, each week one time, started from week one, using a Bruker Optics Minispec NMR analyzer (Billerica, MA) in accordance with the manufacturer's recommendations.
Zhang L., Cai X., Ma F., Qiao X., Ji J., Ma J.A., Vergnes L., Zhao Y., Yao Y., Wu X, & Boström K.I. (2024). Two-step regulation by matrix Gla protein in brown adipose cell differentiation. Molecular Metabolism, 80, 101870.
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7

Quantifying Body Composition by NMR

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Fat mass and lean body mass were determined by NMR (Minispec NMR Analyzer; Bruker). In select experiments, at the time of euthanasia subcutaneous white adipose tissue (WAT) was quantified by determining the weight of the inguinal fat pad, and visceral WAT was quantified by determining the weight of the gonadal fat pad70 (link).
Sacharidou A., Chambliss K., Peng J., Barrera J., Tanigaki K., Luby-Phelps K., Özdemir İ., Khan S., Sirsi S.R., Kim S.H., Katzenellenbogen B.S., Katzenellenbogen J.A., Kanchwala M., Sathe A.A., Lemoff A., Xing C., Hoyt K., Mineo C, & Shaul P.W. (2023). Endothelial ERα promotes glucose tolerance by enhancing endothelial insulin transport to skeletal muscle. Nature Communications, 14, 4989.
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8

Body Composition and Skeletal Measurements

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A Bruker Minispec NMR analyzer (Bruker Corp.) was used to measure body composition, which was determined every month starting 1 day before Tam treatment until the time of dissection (12 or 22 months of age), as previously described (Junnila et al., 2016 (link)). Body length was measured at the time of dissection from the tip of the nose to the anus. Femur length was measured using a digital caliper.
Duran‐Ortiz S., List E.O., Ikeno Y., Young J., Basu R., Bell S., McHugh T., Funk K., Mathes S., Qian Y., Kulkarni P., Yakar S., Berryman D.E, & Kopchick J.J. (2021). Growth hormone receptor gene disruption in mature‐adult mice improves male insulin sensitivity and extends female lifespan. Aging Cell, 20(12), e13506.
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9

Targeted Gene Therapy for Glycogen Storage Disease

Cited 2 times
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All animal studies were conducted under an animal protocol approved by the Eunice Kennedy Shriver National Institute of Child Health and Human Development Animal Care and Use Committee. The rAAV-G6PC-S298C vector was infused into 2-week-old G6pc−/− mice via the retro-orbital sinus as described previously [2 (link)]. Age-matched G6pc+/+ and G6pc+/− mice with indistinguishable phenotype were used as controls. Body composition was assessed using the Bruker Minispec NMR analyzer (Karlsruhe, Germany). Liver samples from control and rAAVG6PC-S298C-treated mice were collected at sacrifice following 12 hours or 24 hours of fast. Hepatic levels of glucose, G6P, lactate, triglyceride, and glycogen were determined as described previously [3 (link)–5 (link)]. Insulin tolerance testing of mice consisted of a 4-hour fast, prior to blood sampling, followed by intraperitoneal injection of an insulin dose of 0.25 IU/kg, and repeated blood sampling via the tail vein for 1 hour.
Zhang L., Lee C., Arnaoutova I., Anduaga J., Starost M.F., Mansfield B.C, & Chou J.Y. (2020). Gene therapy using a novel G6PC-S298C variant enhances the long-term efficacy for treating glycogen storage disease type Ia. Biochemical and biophysical research communications, 527(3), 824-830.
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10

Metabolic Profiling in Murine Liver

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Body composition was assessed using the Bruker minispec NMR analyzer (Karlsruhe, Germany). The presence of HCA nodules in mice was confirmed by histological analysis of liver biopsy samples, using 5 or more separate sections per liver. Blood levels of glucose along with hepatic levels of glucose, triglyceride, lactate, and G6P were determined as described previously [12 , 13 (link)]. Insulin tolerance testing of mice consisted of a 4-hour fast, prior to blood sampling, followed by intraperitoneal injection of insulin at 0.25 IU/kg, and repeated blood sampling via the tail vein for 1 hour [13 (link)].
Kim G.Y., Lee Y.M., Kwon J.H., Cho J.H., Pan C.J., Starost M.F., Mansfield B.C, & Chou J.Y. (2017). Glycogen storage disease type Ia mice with less than 2% of normal hepatic glucose-6-phosphatase-α activity restored are at risk of developing hepatic tumors. Molecular genetics and metabolism, 120(3), 229-234.
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