Luciferase report assay

Manufactured by Promega

Sourced in United States

The Luciferase Reporter Assay is a laboratory tool used to quantify the activity of luciferase, a bioluminescent protein. It provides a sensitive and reliable method to measure gene expression or reporter activity in cells or cell extracts.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Recombinant m csf, by R&D Systems (1 mentions) Mts cytotoxicity assay, by Promega (1 mentions)

Close spelling variants of this product

Dual-Luciferase Report Assay Kit Luciferase assay report kit Luciferase Report Assay System Dual-Luciferase Report Assay Dual-Luciferase Report Assay System Luciferase assay

2 protocols using luciferase report assay

Investigating miR-182 Regulation of TSHR and ELF3

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TargetScan (http://www.targetscan.org/vert_72/) predicted the binding sites of miR-182 with TSHR. The targeting relationship was then confirmed with dual- luciferase reporter gene assay. The 3’-UTR cDNA sequences (including the binding sites to miR-182) were cloned into the pGL3 luciferase reporter gene (Genepharma, Shanghai, China). MiR-182 mimics or NC and TSHR-WT/MUT mimics were co-transfected into cells for 48 h. Luciferase activity was verified following the luciferase report assay (Promega, WI, USA) procedure.
The binding sites of ELF3 to miR-182 promoters were speculated using JASPAR (https://jaspar.genereg.net/). Oe-ELF3 or oe-NC plasmids, reporter gene plasmids and internal reference plasmids were transfected into cells for 48 h. Luciferase activity was verified according to the luciferase report assay (Promega) procedure.

Wang S., Chen L, & Xu B. (2023). ELF3-induced miR-182 inhibits adipogenic differentiation in Graves’ orbitopathy by targeting thyrotropin receptor. Central-European Journal of Immunology, 47(4), 308-322.

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Asperpyrone A Modulates Osteoclastogenesis

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Asperpyrone A, with a purity >99%, was obtained from Professor RenXiang Tan in Nanjing University's (China). DMSO (dimethylsulphoxide), Trizol reagent, FBS (foetal bovine serum), a‐MEM (alpha‐modified minimal essential medium) and Ca²⁺ oscillations assay were purchased from Thermo Fisher Scientific. Recombinant M‐CSF was produced from R&D Systems, and RANKL protein (GST‐rRNAKL) was expressed and purified as previously described.12 The MTS cytotoxicity assay and luciferase report assay were purchased from Promega. The antibodies of phosphorylated ERK (p‐ERK), NFATc1, V‐ATPase‐d2, c‐fos, IκB‐α and JNK were obtained from Santa Cruz and the antibodies of P38, phosphorylated P‐38 (P‐P38), phosphorylated JNK(p‐JNK), c‐fos and ERK from Cell Signaling Technology.

Chen X., Wang C., Qiu H., Yuan Y., Chen K., Cao Z., Xiang Tan R., Tickner J., Xu J, & Zou J. (2019). Asperpyrone A attenuates RANKL‐induced osteoclast formation through inhibiting NFATc1, Ca2+ signalling and oxidative stress. Journal of Cellular and Molecular Medicine, 23(12), 8269-8279.

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