Donkey anti sheep tritc

Manufactured by Jackson ImmunoResearch

Sourced in Cameroon

Donkey anti sheep-TRITC is a secondary antibody conjugated with the fluorescent dye TRITC (Tetramethylrhodamine). It is designed to detect and visualize sheep primary antibodies in immunological applications such as immunofluorescence, flow cytometry, and Western blotting.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Avidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti avidin fitc, by Merck Group (1 mentions) Anti digoxigenin rhodamine, by Roche (1 mentions) Vectashield, by Vector Laboratories (1 mentions) Rabbit α synuclein, by Cell Signaling Technology (1 mentions) Prolong gold, by Cell Signaling Technology (1 mentions) Donkey anti rabbit fitc, by Jackson ImmunoResearch (1 mentions) Zen software, by Zeiss (1 mentions)

2 protocols using donkey anti sheep tritc

Two-step DNA Probe Visualization

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DNA probe signals were captured by two-steps system detection of biotin-labeled probe with avidin–AlexaFluor488 (Molecular Probes; dilution 1:100; final concentration: 10 µg/ml) and anti-avidin-FITC (Sigma; dilution 1:100; final concentration: 20 µg/ml), and two-steps system detection of digoxigenin-labeled probe with anti-digoxigenin–rhodamine produced in sheep (Roche; dilution 1:100; final concentration: 2 µg/ml) and secondary antibody donkey anti sheep-TRITC (Jackson ImmunoResearch; dilution 1:100; final concentration: 10 µg/ml). DNA was counterstained using Hoechst 33342 (Molecular Probes), and samples were mounted with Vectashield (Vector Laboratories).

Trzaskoma P., Ruszczycki B., Lee B., Pels K.K., Krawczyk K., Bokota G., Szczepankiewicz A.A., Aaron J., Walczak A., Śliwińska M.A., Magalska A., Kadlof M., Wolny A., Parteka Z., Arabasz S., Kiss-Arabasz M., Plewczyński D., Ruan Y, & Wilczyński G.M. (2020). Ultrastructural visualization of 3D chromatin folding using volume electron microscopy and DNA in situ hybridization. Nature Communications, 11, 2120.

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Immunofluorescence Analysis of α-Synuclein in SN

Cited 1 time

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Immunofluorescence was performed on every 6^th section in the SN using polyclonal antibodies for sheep TH (1:1000, Abcam, Cambridge, MA) and rabbit α-synuclein (1:250, Cell Signaling, Danvers, MA) as previously described [36 (link)]. Non-specific binding was blocked for one hour using 10% normal donkey serum in phosphate-buffered saline (PBS) + 0.3% Triton-X-100, followed by primary antibody incubation overnight. The next day, sections were incubated for one hour in secondary antibody, donkey-anti-rabbit-FITC (1:200) and donkey-anti-sheep-TRITC (1:50, Jackson ImmunoResearch, West Grove, PA), followed by 10 minutes in DAPI solution, then rinsed in PBS. Sections were mounted and coverslipped using ProLong Gold (Cell Signaling). Z-stack images of TH/DAPI were taken using a Zeiss LSM 880 Quasar inverted laser scanning confocal/multiphoton microscope with 63x/1.4NA planapochromat oil immersion objective using ZEN software (Zeiss, Thornwood, NY).

Farrand A.Q., Helke K.L., Gregory R.A., Gooz M., Hinson V.K, & Boger H.A. (2017). Vagus nerve stimulation improves locomotion and neuronal populations in a model of Parkinson’s disease. Brain stimulation, 10(6), 1045-1054.

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