Mouse anti acsl4

The following primary antibodies and dilutions were used for immunofluorescence
studies: mouse anti-Flag (Sigma-Aldrich, F3165) (1:1000), rabbit anti-TOM20 (Santa-Cruz,
sc11415) (1:1000), rabbit anti-PMP70 (Sigma-Aldrich, SAB4200181) (1:1000), mouse
anti-p230 (BD Biosciences, 611281) (1:1000), rabbit anti-Rab7 (Abcam, ab137029)
(1:1000), rat anti-Calnexin (Biolegend, 699401) (1:1000) and mouse anti-Neurofilament H
(Biolegend, 835801). Donkey anti-mouse, Goat anti-mouse IgG1, Goat anti-mouse IgG2a,
Goat anti-rabbit and Goat anti-rat, Alexa Fluor 488, 568 or 647 were used as secondary
antibodies (1:1000) (Invitrogen).
The following primary antibodies and dilutions were used for immunoblot analysis: mouse
anti-Flag (Sigma-Aldrich, F3165) (1:1000), mouse anti-VDAC1 (Abcam, ab14734) (1:1000),
rabbit anti-TMEM63C (Abcam, ab203486) (1:500), rabbit anti-Pex14 (Proteintech,
10594-1-AP) (1:1000), rabbit anti-Calnexin (Proteintech, 10427-1-AP) (1:1000), mouse
anti-Tubulin (Santa-Cruz, sc23948) (1:1000), rabbit anti-VAPB (Atlas, HPA013144) (1:500)
and mouse anti-ACSL4 (Santa-Cruz, sc-365230) (1:1000). Horseradish peroxidase-conjugated
anti-rabbit and anti-mouse IgG (GE Healthcare) were used as secondary antibodies
(1:3000).

The following primary antibodies were used: rabbit anti-Nogo-A/RTN4 (AHP1799, Biorad, Feldkirchen, Germany), mouse anti-TOM20 (Santa Cruz, CA, USA, sc-17764), mouse anti-SIGMA1R (Santa Cruz, sc-137075), rat anti-SIGMA1R (Merck, Darmstadt, Germany, ICR-SIG1R-A), mouse anti-ACSL4 (Santa Cruz, sc-365230), mouse anti-Myc (Cell Signaling, Danvers, MA, USA, 2276), rabbit anti-HA (Sigma-Aldrich, St. Louis, MO, USA, H6908), rat anti-HA (Roche, Basel, Switzerland, 3F10), rabbit anti-VLGR1, raised against the against the C-terminus of murine VLGR1 (amino acids 6198–6307) and previously characterized in [9 (link),14 (link)], rabbit anti-α-Tubulin (Abcam, Cambridge, UK, DM1A), rabbit anit-COX IV (NEB, Ipswich, MA, USA, 3E11), rabbit anti-GFAP (Dako Agilent, Santa Clara, CA, USA, Z0334), rat anti-RFP (Chromotek, Planegg, Germany, 5F8), mouse anti-CLIMP63, (Enzo Life Sciences, G1/296), goat anti-Pericentrin 2 (Santa Cruz, C-16), mouse anti-Arl13b (Abcam, Cambridge, UK, ab136648), goat anti-Centrin 2 [15 (link)]. Secondary antibodies conjugated Alexa 488, Alexa 555, Alexa 568 or Alexa 647 were purchased from Molecular Probes (Life Technologies, Darmstadt, Germany) or Rockland Inc. (Gilbertsville, PA, USA). Nuclear DNA was stained with DAPI (4′,6-diamidino-2-phenylindole, 1 mg/mL: Sigma-Aldrich).