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Nrf2 shrna sc 37030 sh

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Nrf2 ShRNA (sc-37030-SH) is a short hairpin RNA (shRNA) construct targeting the human nuclear factor erythroid 2-related factor 2 (Nrf2) gene. It is designed to knock down the expression of the Nrf2 gene.

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Lab products found in correlation

Neon transfection system, by Thermo Fisher Scientific (2 mentions) Control shrna sc 108060, by Santa Cruz Biotechnology (2 mentions) Pegfp c1 plasmid vector, by Takara Bio (1 mentions)

2 protocols using nrf2 shrna sc 37030 sh

1

Eukaryotic Expression Plasmids and Transfection

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pEGFP-C1 plasmid vector for eukaryotic expression was purchased from Clontech (Palo Alto, CA, USA). pGFP-Klf9 (pCMV-AC-GFP-Klf9) plasmid was purchased from OriGene (RG210147, OriGene Technologies, Inc., Rockville, MD, USA). pcDNA3-EGFP-C4-Nrf2 (pEGFP-Nrf2) plasmid were purchased from Addgene (Watertown, MA, USA). Control shRNA (sc-108060) and Nrf2 ShRNA (sc-37030-SH) plasmids were purchased from Santa Cruz Biotechnology. For experimentation, LECs were transfected with Control ShRNA or Nrf2 ShRNA using the Neon Transfection System (Invitrogen, Waltham, MA, USA).
Chhunchha B., Kubo E, & Singh D.P. (2022). Switching of Redox Signaling by Prdx6 Expression Decides Cellular Fate by Hormetic Phenomena Involving Nrf2 and Reactive Oxygen Species. Cells, 11(8), 1266.
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2

Stable Nrf2 knockdown in SRA-hLECs

Cited 3 times
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Control shRNA (sc-108060) and Nrf2 shRNA (sc-37030-SH) plasmids were purchased from Santa Cruz Biotechnology, Dallas, TX, USA. SRA-hLECs were transfected with Control shRNA and Nrf2 shRNA. To make stable cell lines transfectants were selected with the selection marker puromycin. SRA-hLECs were transfected by using the Neon Transfection System (Invitrogen, Waltham, MA, USA) as described in our published protocol [21 (link),74 (link),81 (link)].
Chhunchha B., Kubo E, & Singh D.P. (2022). Obligatory Role of AMPK Activation and Antioxidant Defense Pathway in the Regulatory Effects of Metformin on Cellular Protection and Prevention of Lens Opacity. Cells, 11(19), 3021.
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