Supernatants were obtained by incubating 25-30 mg of 11 (4 NSND, 7 SD) tumor fragments in 250 µL of RPMI medium supplemented with glutamine and penicillin/streptomycin for 24 h. Sera samples were obtained from 26 OSCC patients (11 NSND and 15 SD) and 18 HD (5 NSND, 13 SD). Samples were frozen at -80°C. Cytokine levels were measured in tumor supernatants and sera by a cytometric bead array (CBA) assay using the human Th1/Th2/Th17 Cytokine Kit (BD Biosciences, Le Pont de Claix, France). Data were collected using a LSRII flow cytometer (Becton Dickinson) and then processed for analysis with the FCAP Array software (version 3.0, BD Biosciences).
Human th1 th2 th17 cytokine kit
Manufactured by BD
Sourced in United States
The BD Human Th1/Th2/Th17 Cytokine Kit is a multiplex assay designed to quantitatively measure the levels of multiple cytokines associated with T-helper cell subsets. The kit provides a tool for the simultaneous detection and analysis of cytokines critical for Th1, Th2, and Th17 cell function.
Automatically generated - may contain errors
Lab products found in correlation
Fcap array software, by BD (3 mentions)
Facsarray bioanalyzer, by BD (3 mentions)
Facscanto 2 flow cytometer, by BD (2 mentions)
Fcap array, by BD (2 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Facscanto flow cytometer, by BD (1 mentions)
Enzyme immunoassay, by R&D Systems (1 mentions)
24 well plate, by Thermo Fisher Scientific (1 mentions)
Facscanto 2 cytometer, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Facsverse, by BD (1 mentions)
Facsuite, by BD (1 mentions)
Facsverse flow cytometer, by BD (1 mentions)
Human il 1β quantikine elisa kit, by R&D Systems (1 mentions)
Bead ruptor 24, by Omni International (1 mentions)
Facsdiva software, by BD (1 mentions)
15 protocols using human th1 th2 th17 cytokine kit
1
Cytokine Profiling in OSCC Tumors
2
Multiplex Cytokine Profiling in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Production of IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-15, IL-23, IL-33, CCL-10 (IP-10), CCL-11 (eotaxin-1), IFN-g, TNF-α, and brain-derived neurotrophic Factor (BDNF) were investigated in plasma using enzyme immunoassay (R&D Systems®, San Diego, CA, USA), following the manufacturer’s instructions and expressed in pg/mL, the material used was stored in a −80° freezer from collection and underwent two thawing cycles. The cytometric bead array (CBA) was used to measure cytokines and chemokines in the cell culture supernatant following the manufacturer’s instructions to address the production of the following chemokines CXCL8/IL-8, CCL5/RANTES, CXCL9/MIG, CCL2/MCP-1, and CXCL10/IP-10. The Human Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA) was also used, and the acquisition was performed on a BD FACSCanto™ flow cytometer (BD Immunocytometry Systems, BD Biosciences, San Jose, CA, USA). The concentration of the sample was estimated by comparing the fluorescence of PE obtained from the standard curve obtained by serial dilution of recombinant human chemokines and cytokines. Results were analyzed by 5-Parameter Logistic Regression in FCAP array software and expressed in pg/mL.
3
Cytokine Production by Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MDS-MSC and control-MSC were seeded in duplicates into 24-well plates (Nunc, Naperville, IL), at a concentration of 30,000 cells/cm2 with 2 mL of cultured medium (DMEM low glucose supplemented with 20% of FBS, 2 mM L-glutamine and antibiotics). The cells were treated with one of the following: 5-Azacytidine 1 µM, 5-Azacytidine 5µM or left without treatment as controls. After 96 h of culture, the supernatant was removed and cryopreserved at −80 °C for further analysis. The MSC were detached with trypsine and then the cell viability was assessed by flow cytometry, using iodine propidium.
The in vitro MSC production of cytokines was evaluated in the collected supernatants. The cytokines were measured, using a cytometric bead array (CBA) for human Th1/Th2/Th17 cytokines (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, USA), following the manufacturer instructions. We measured the IL-2, IL-4, IL-6, IL-10, INF-γ, TNF-α and IL-17A. The data were acquired with a FACS Canto II cytometer, using the digital instrument CBA template (BD) for FACS DIVA. The data were analyzed by the FCAP Array software (BD).
The in vitro MSC production of cytokines was evaluated in the collected supernatants. The cytokines were measured, using a cytometric bead array (CBA) for human Th1/Th2/Th17 cytokines (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, USA), following the manufacturer instructions. We measured the IL-2, IL-4, IL-6, IL-10, INF-γ, TNF-α and IL-17A. The data were acquired with a FACS Canto II cytometer, using the digital instrument CBA template (BD) for FACS DIVA. The data were analyzed by the FCAP Array software (BD).
4
Cytokine Profiling in Neurological Diseases
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum and CSF samples from patients and controls were collected and stored at −75°C until processing. Determination of serum and CSF levels of the cytokines IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, and IL-17A was performed on a BD FACSArray Bioanalyzer using the Cytometric Bead Array (CBA) assay (human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, San Diego, USA). Serum and CSF levels of TGF-β1 were measured by ELISA (R&D Systems Quantikine TM, Minneapolis, MN, USA). The data were analyzed using the CurveExpert V1.4. Cytokine ratios (Th1/Th2, Th1/Th17, Th17/Th2, Type-1/Type-2, IFN-γ/IL-10, and IL-17A/IL-10) were also calculated (Table 1 ).
5
Plasma Cytokine Profiling in T1D
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood (10 mL) was collected from T1D patients and controls and plasma-EDTA was isolated by centrifugation at 1,372 g, for 10 min, 4°C. Cytokine detection was performed by cytometric bead array (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, CA, USA). Plasma levels of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and IFN-γ were determined by flow cytometer FACSCanto™ II (BD Biosciences). Analyses was performed by BDFCAP array™ software and data were expressed by conversion of the median fluorescence intensity in picogram per milliliter.
6
Multiplex Cytokine Profiling in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After peripheral blood collection (8 mL) in gel tubes with clot activator, samples were incubated for 50 min and then centrifuged at 1372 g for 5 min, 25 °C. Isolated serum samples were stored until cytokine determination. Cytokine detection was performed by using a cytometric bead array (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, Franklin Lakes, NJ, USA). Serum levels of IL-2, IL-4, IL-6, IL-10, IL-17A, tumor necrosis factor (TNF), and IFN-γ were determined by flow cytometer FACSCanto™ II (BD Biosciences). Analyses were performed by BDFCAP array™ software, and data were expressed in pg/mL.
7
Cytokine Profiling in Adenocarcinoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For evaluating changes in cytokine levels in patients with adenocarcinoma during treatment, the Human Th1/Th2/Th17 Cytokine Kit (#560484, BD Bioscience, San Jose, CA, USA) was used according to the manufacturer’ instructions. IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17A were quantified. Data were acquired through cytometry using the FACSCanto II and were analyzed using the FCAPArray software v. 3.0 (Softflow, Pécs, Hungary).
8
Multiplex Cytokine Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma concentrations of IL-2, -4, -6, -10, -17A, TNF-α, and IFN-γ were measured on a FACSVerse flow cytometer (BD Biosciences, Heidelberg, Germany) using a multiplex assay (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, Heidelberg, Germany) according to the manufacturer’s instructions.
9
Simultaneous Quantification of Cytokines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Because of IL-6 and IL-10 levels around the detection limit of the standard set, cytokine plasma levels, including IL-2, IL-4, IL-6, IL-10, IL-17A, TNFα, and IFNγ, were simultaneously quantified with the Human Th1/Th2/Th17 Cytokine Kit and additional Enhanced Sensitivity Flex Set IL6/IL10 (all BD Biosciences Pharmingen, San Diego, CA), according to the instruction manual. Data acquisition and analysis was performed with a FACSVerse flow cytometer, using FACSuite and FCAP Array software (BD Biosciences Pharmingen).
10
Cytokine Quantification in T2D Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood (10 mL) was collected from T2D patients and controls and plasma-EDTA was separated by centrifugation at 1,372 g, for 10 min, 4°C. Cytokine quantification was performed by cytometric bead array (Human Th1/Th2/Th17 Cytokine Kit, BD Biosciences, CA, USA). Plasma levels of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF, and interferon-gamma (IFN-γ) were detected by flow cytometer FACSCanto™ II (BD Biosciences). The analyses were performed by using BDFCAP array™ software and results were expressed by conversion of the median fluorescence intensity in picograms per milliliter.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!