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Sea133ra

Manufactured by Cloud-Clone
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Sourced in China, Germany

The SEA133Ra is a laboratory equipment designed for conducting scientific experiments and research. It serves as a core component in various laboratory settings. The product specification and technical details are available upon request.

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Lab products found in correlation

Sea079ra, by Cloud-Clone (3 mentions) Elisa reader, by RayBiotech (1 mentions) Sea563ra, by Cloud-Clone (1 mentions) Sea056ra, by Cloud-Clone (1 mentions) A001 1 2, by Nanjing Jiancheng (1 mentions) A006 1 1, by Nanjing Jiancheng (1 mentions) A003 1 2, by Nanjing Jiancheng (1 mentions) A044 1 1, by Nanjing Jiancheng (1 mentions) Glutathione (gsh), by Nanjing Jiancheng (1 mentions)

6 protocols using sea133ra

1

ELISA-Based Serum TNF-α Quantification

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Serum TNF‐α levels were detected using an ELISA kit (SEA133Ra, Cloud‐Clone Corp., Wuhan, China) according to the manufacturer’s instructions. Absorbance was measured at 450 nm against a blank using an ELISA reader (RayBiotech, Canada). Assay results were expressed as pg ml‐1.
Khakisahneh S., Zhang X., Nouri Z, & Wang D. (2021). Cecal microbial transplantation attenuates hyperthyroid‐induced thermogenesis in Mongolian gerbils. Microbial Biotechnology, 15(3), 817-831.
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2

Cytokine Levels in Serum and Culture

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The levels of IL-1β (Cloud-Clone Corp, SEA563Ra, Wuhan, China), TNF-α (Cloud-Clone Corp, SEA133Ra), IL-10 (Cloud-Clone Corp, SEA056Ra), IL-6 (Cloud-Clone Corp, SEA079Ra) in serum and cell culture supernatant were detected according to the instructions.
Fu Z., Zhang Z., Wu X, & Zhang J. (2020). Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway. BioMed Research International, 2020, 9121894.
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3

Cytokine and Oxidative Stress Measurements

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Rat-specific ELISA kits were applied to determine hepatic and medium tumor necrosis factor α (TNF-α, SEA133Ra, USCN, Cloud-Clone Corp., Wuhan, China), monocyte chemokine protein-1 (MCP-1, SEA087Mi, USCN, Cloud-Clone Corp., Wuhan, China), and hepatic interleukin-6 (IL-6, SEA079Ra, USCN, Cloud-Clone Corp., Wuhan, China) and levels according to the manufacturer's instructions. Absorbance all measured at 450 nm. Hepatic 8-isoprostane level was determined using Cayman: 8-Isoprostane EIA Kit (number 516351, Cayman Chemical, USA) according to the manufacturer's instruction. The absorbance was measured at a wave length of 420 nm.
Zhang Y., Yuan D., Yao W., Zhu Q., Liu Y., Huang F., Feng J., Chen X., Huang Y., Chi X, & Hei Z. (2016). Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity, 2016, 3919627.
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4

Cytokine and Oxidative Stress Evaluation

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Cytokines was correlated tightly with the occurrence of the infection, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1), while myeloperoxidase (MPO), malondialdehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) were correlated with oxidative stress reaction, which was often occurred in UC. So here we measured this cytokinesis to evaluate the efficacy of the treatment. IL-6 (SEA079Ra; Cloud-Clone Corp) and TNF-α (SEA133Ra; Cloud-Clone Corp) were quantified using commercially available ELISA kits. Standard curves are shown in Supplementary Figure S1D,E. MPO (A044-1-1; Nanjing Jiancheng Bioengineering Institute), GSH (A006-1-1; Nanjing Jiancheng Bioengineering Institute), MDA (A003-1-2; Nanjing Jiancheng Bioengineering Institute) and T-SOD (A001-1-2; Nanjing Jiancheng Bioengineering Institute levels in sera were determined by ELISA assay kits according to the manufacturer’s instructions.
Rong Y., Hong G., Zhu N., Liu Y., Jiang Y, & Liu T. (2021). Photodynamic Therapy of Novel Photosensitizer Ameliorates TNBS-Induced Ulcerative Colitis via Inhibition of AOC1. Frontiers in Pharmacology, 12, 746725.
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5

Cytokine and Protein Profiling in BALF and Serum

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BALF and serum samples were collected from each group as described above. According to the manufacturer’s protocols, using ELISA kit to detect the content of IL-8 (ABIN2535650, 4A Biotech, Germany), TNF-α (SEA133Ra, Cloud-Clone, China) in BALF and serum of rats. We also detect the content of Hsp90α (SED523Mi, Cloud-Clone, China) and MLKL (SER645Ra, Cloud-Clone, China) in serum of rats.
Feng N., Han X., Peng D., Geng F., Li Q., Pan C., Wang H., Pan Y, & Tan L. (2024). P. gingivalis alters lung microbiota and aggravates disease severity of COPD rats by up-regulating Hsp90α/MLKL. Journal of Oral Microbiology, 16(1), 2334588.
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6

Measuring TNF-α Expression in Cortical Tissue after ICH

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After deep anesthesia by sodium pentobarbital (45 mg/kg i.p.), the cortex tissue around right ICH was extracted. Homogenates of cortical tissue around the injury site were taken, centrifuged (12 000 × g for 20 min at room temperature), and the supernatant obtained. Expression of tumor necrosis factor-α (TNF-α) was measured using the kits (SEA133Ra, Cloud-Clone, Wuhan, China).
Luo W., Bu W., Chen H., Liu W., Lu X., Zhang G., Liu C., Li X, & Ren H. (2024). Electroacupuncture reduces oxidative stress response and improves secondary injury of intracerebral hemorrhage in rats by activating the peroxisome proliferator-activated receptor-γ/nuclear factor erythroid2-related factor 2/γ-glutamylcysteine synthetase pathway. Neuroreport, 35(8), 499-508.
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