The A2780 cell line was purchased from American Type Culture Collection (ATCC), and SAHA was purchased from Selleckchem (Houston, TX, USA). Primary antibodies against Ac-H2A, Ac-H2B, Ac-H3, Ac-H4, HDAC2, HDAC3, HDAC4, DNMT3A, PRMT1, SUV39H1, MDMX, p53, p21WAF1/CIP1, p27Kip1, AURKB, CDC25C, GADD45A (1:1000) and Alexa Fluor 488 dye were purchased from Cell Signaling Technology (Danvers, MA, USA). The MDM2 antibody (1:500) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). β-actin (1:5000), secondary antibodies (anti-rabbit, anti-mouse), horseradish peroxidase (HRP) conjugate, and dimethyl sulfoxide (DMSO) were purchased from Sigma Aldrich (St. Louis, MO, USA). Nitrocellulose membrane (NC) (0.45 µm) was purchased from Amersham (GE Healthcare Life Sciences, Marlborough, MA, USA) and KPL LumiGlo Reserve chemiluminescent substrate was purchased from SeraCare Life Sciences (Milford, MA, USA).
Suv39h1
Manufactured by Cell Signaling Technology
Sourced in United States
SUV39H1 is a histone methyltransferase that catalyzes the trimethylation of lysine 9 on histone H3 (H3K9me3). This epigenetic mark is associated with the formation of heterochromatin and gene silencing. SUV39H1 is a core component of the chromatin remodeling machinery and plays a critical role in regulating gene expression and chromosomal structure.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hdac3, by Cell Signaling Technology (1 mentions)
A2780 cell line, by American Type Culture Collection (1 mentions)
β actin, by Merck Group (1 mentions)
P27kip1, by Cell Signaling Technology (1 mentions)
P21 waf1 cip1, by Cell Signaling Technology (1 mentions)
Cdc25c, by Cell Signaling Technology (1 mentions)
Gadd45a, by Cell Signaling Technology (1 mentions)
Aurkb, by Cell Signaling Technology (1 mentions)
Ac h3, by Cell Signaling Technology (1 mentions)
Ac h4, by Cell Signaling Technology (1 mentions)
Prmt1, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 dye, by Cell Signaling Technology (1 mentions)
Mdm2 antibody, by Santa Cruz Biotechnology (1 mentions)
Horseradish peroxidase hrp conjugate, by Merck Group (1 mentions)
Hdac2, by Cell Signaling Technology (1 mentions)
Hdac4, by Cell Signaling Technology (1 mentions)
Dnmt3a, by Cell Signaling Technology (1 mentions)
Protein a g agarose, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 protocols using suv39h1
1
Histone Modification Profiling in A2780 Cells
2
Hsp90 Chaperone Complex Components
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Roswell park memorial institute (RPMI) 1640 medium, fetal bovine serum (FBS) and trypsin were obtained from Gibco Invitrogen (Grand Island, NY, USA). Antibodies used to detect C-ABL, AML1-ETO, c-Raf, Akt, phosphorylated-Akt, phosphorylated-Erk, Erk, BCR-ABL, heat shock protein 90 (Hsp90), HOP, pp5, p23, Hsp70, SUV39H1, HOP, and me-H3K9 were purchased from Cell Signaling Technology (Boston, MA, USA). Primary antibodies (against Tubulin, Actin and GAPDH) and secondary antibodies were purchased from HuaBio (Hangzhou, China). Anti-Hsp90 antibody and protein A/G agarose were purchased from Santa Cruz Biotechnology, Inc (Dallas, TX, USA). Anti-Hsp90 (AC88) antibody, anti-p23 antibody and full-length Hsp90α (FL-Hsp90α) were purchased from Abcam (Cambridge, UK). 17-Allyl-17-demethoxygeldanamycin (17-AAG) was purchased from Apollo Scientific Limited (Stockport, UK). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), TPCK-treated trypsin, adenosine 5′-triphosphate (ATP)-agarose and alcohol dehydrogenase equine (ADH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell lysis buffer for Western blotting (WB) and immunoprecipitation (IP), PMSF, MG132, a BCA Protein Assay Kit and IgG antibody were purchased from Beyotime Institute of Biotechnology (Shanghai, China).
3
Western Blot Analysis of SUV39H1 and H3K9me3
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Cellular proteins were subjected to 10–12% SDS-polyacrylamide gel electrophoresis and blotted onto polyvinylidene difluoride membrane. Western blot was performed as previously described24 (link). We employed antibodies against SUV39H1(Cell Signalling, Hitchin, UK), H3K9me3(Novus Biologicals, Littleton, CO, USA), and β-actin(Abcam, Cambridge, UK). The intensities of immunoreactive bands were quantified using Image Gauge software(Fuji Film, Tokyo, Japan).
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