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3 protocols using calcium sulfate

1

Synthesis of Transition Metal Sulfate Solutions

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Manganese sulfate, ammonium sulfate, ferric sulfate, cobalt sulfate, and nickel sulfate were purchased from Aladdin Industrial Corp. (Shanghai, China). Copper sulfate, zinc sulfate, magnesium sulfate, calcium sulfate, sodium silicate, arsenic acid and sulfuric acid were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). All the reagents were of analytical grade. The simulated electrolyte was prepared by dissolving analytical-grade manganese sulfate and other reagents in ultrapure water. sulfuric acid was used to adjust the pH. Different solutions were prepared in 100.0 mL glass volumetric flask.
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2

Diverse Biological Reagents Acquisition

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Neutral protease, papain, chitosan, bovine insulin, myoglobin, and tryptophan were purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). Tryptone, yeast extract, Agar, NaCl, glycerin, K2HPO4·3H2O (AR), sorbitol, MgSO4·7H2O (AR), trisodium citrate, MgCl2 (AR), kanamycin, ampicillin, peptone, maltose, sodium acetate, calcium sulfate, acetic acid, Triton X-100, phosphate-buffered saline, normal saline, trichloroacetic acid, and other reagents (AR) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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3

Synthetic Wastewater Treatment Protocol

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4-Methyl-octylphenol, 4-tert-butyl-octylphenol, 4-tert-octylphenol, chloroacetic acid, dimethyl sulfoxide (DMSO) and NaOH were purchased from Chendu Xiya Reagent Chemical Technology Co., Ltd. Concentrated hydrochloric acid, oxalic acid, calcium hydroxide, sodium phosphate, ammonium chloride, calcium sulfate and magnesium sulfate were purchased from Sino-pharm Chemical Reagent Co., Ltd., China. All other chemicals were used without further purification.
Real wastewater sample was collected from the RE industry in Inner Mongolia, China. The typical compositions of the wastewater are listed as follows, CaSO4 0.0113 mol L−1, MgSO4 0.124 mol L−1 and (NH4)2SO4 0.325 mol L−1. In order to reduce the influence of impurities, the synthetic wastewater samples containing CaSO4, MgSO4 and (NH4)2SO4 were prepared and used in the parts of mechanism research.
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