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Ab 439690

Manufactured by Merck Group
Sourced in United States

The AB_439690 is a laboratory equipment product manufactured by Merck Group. It is designed for specialized scientific applications. The core function of this product is to assist in conducting research and analysis in a laboratory setting. No further details about the intended use or specifications of this product can be provided in an unbiased and factual manner.

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2 protocols using ab 439690

1

Intracellular Signaling Pathway Analysis

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Dopamine, (−) quinpirole, isoproterenol, forskolin, 4-amino-5-(4-chlorophenyl)-7-(dimethyl ethyl) pyrazolo [3,4-d] pyrimidine (PP2), wortmannin, triciribine, agarose beads coated with monoclonal antibodies against FLAG epitope, rabbit anti-FLAG M2 antibodies (AB_439687), rabbit antibodies against green fluorescent protein (GFP)(AB_439690), and HA antibodies (AB_2610070) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Goat anti-mouse Alexa Fluor® 555 (AB_2535844), anti-rabbit Alexa Fluor® 555 (AB_2535849), anti-rabbit HRP-conjugated secondary antibodies (AB_10960844), and DAPI were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies to β-actin (AB_10950489), lamin b1 (AB_10896336), phospho-T308-Akt (AB_331170), phospho-S473-Akt (AB_331163), PDK1 (AB_2236832), phospho-S241-PDK1 (AB_2161134), phospho-Y416-Src (AB_331697), and arrestin2/3 (AB_10547883) were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). Antibodies to USP33 (AB_2213243), importin β1 (AB_2133993), phospho-ERK1/2 (AB_1125801), and ERK2 (AB_2141292) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti-mouse HRP-conjugated secondary antibodies (AB_10015289) were obtained from Jackson ImmunoResearch (West Grove, PA, USA).
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2

Biochemical Assays for Protein Interactions and Modifications

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PMA, leptomycin A (LMB), agarose beads coated with monoclonal antibodies against FLAG epitope, rabbit anti-FLAG M2 antibodies (AB_439687), rabbit antibodies against GFP (AB_439690), and HA antibodies (AB_2610070) were purchased from Sigma-Aldrich Chemical Co. Latrunculin A (LatA) was obtained from Cayman Chemical. Mouse monoclonal antibodies against CHC (AB_397865) were obtained from BD Bioscience. Rabbit antibodies against rictor (AB_2179961), phosphor-PKCα/βII (Thr638/641) (AB_2284224), K48-linkage specific polyubiquitin (AB_10557239), and K63-linkage specific polyubiquitin rabbit mAb (HRP conjugate) antibody (AB_2798064) were purchased from Cell Signaling Technology. Anti-rabbit HRP-conjugated secondary antibodies (AB_2533967) and phospho-PKCβ1&2 (Thr500) (AB_2533805) were obtained from Thermo Fisher Scientific, and anti-mouse HRP-conjugated secondary antibodies (AB_10015289) were purchased from Jackson ImmunoResearch Laboratories, Inc. Mdm2 E3 ligase inhibitor and antibodies against Mdm2 (AB_627920) were obtained from Santa Cruz Biotechnology and torin1 from Selleck Chemicals. Alexa Fluor 594–conjugated anti-rabbit (AB_142057) and anti-mouse (AB_141593) antibodies were from Molecular Probes. [3H]-sulpiride was purchased from Perkin Elmer Life Sciences.
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