Goat anti lamin b c 20

Immunoblotting for HIV-1 Gag/CA p24 was described previously (63 (link)). Immunoblotting for Rev was performed with primary antibody sheep anti-Rev (1:4,500, no. H6006; US Biological Life Sciences) and secondary antibody rabbit anti-goat Ig/HRP (1:5,000, no. P0160; Dako). Immunoblotting for myc-tagged proteins and for GFP was performed using primary antibodies monoclonal mouse anti-myc tag (1:8,000, no. 2276; Cell Signaling Technology) and monoclonal mouse anti-GFP (1:500, no. sc-9996; Santa Cruz) in combination with the secondary antibody goat anti-mouse IgG (1:5,000, no. 115-035-146; Dianova). Immunoblotting for α-tubulin was performed using primary antibody polyclonal rabbit anti-α-tubulin antiserum (1:4,000, no. 600-401-880; Rockland), and secondary swine anti-rabbit Ig/horseradish peroxidase (HRP) (1:5,000, no. P0217; Dako). In experiments shown in Fig. 1B, 6A, and 6C, anti-α-tubulin blotting was conducted after stripping the blotted anti-CA and anti-myc membranes with stripping buffer (no. 2504; Millipore). The membranes were then reblocked and subsequently reblotted with antibodies as described above. Immunoblotting for LaminB was performed with primary antibody goat anti-LaminB (C-20) (1:500, no. sc-6216; Santa Cruz Biotechnology) and secondary antibody rabbit anti-goat Ig/HRP (1:5,000, no. P0160; Dako).