Healthy peripheral whole blood

Manufactured by STEMCELL

Healthy peripheral whole blood is a laboratory product that provides a source of human peripheral blood cells. It is collected from healthy adult donors and is suitable for a variety of in vitro applications.

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Lab products found in correlation

Human nk cell isolation kit, by Miltenyi Biotec (2 mentions) Ficoll paque, by GE Healthcare (2 mentions)

2 protocols using healthy peripheral whole blood

Isolation and Cultivation of NK Cells

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Human PBMCs were isolated from healthy peripheral whole blood (STEMCELL Technologies) by using a gradient method with the Ficoll-Paque (GE Healthcare), and frozen for later use. Cryopreserved PBMCs were cultured in RPMI complete medium supplied with 30 units/ml of human recombinant IL-2 for three days, and the CD56⁺CD3⁻ NK cells were isolated by using the human NK cell isolation kit (Miltenyi Biotec) following the manufacturer's instructions.

Zhou D., Wu Z., Park J.G., Fiches G.N., Li T.W., Ma Q., Huang H., Biswas A., Martinez-Sobrido L., Santoso N.G, & Zhu J. (2022). FACT subunit SUPT16H associates with BRD4 and contributes to silencing of interferon signaling. Nucleic Acids Research, 50(15), 8700-8718.

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Isolation and Activation of Human NK Cells

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Human PBMCs were isolated from healthy peripheral whole blood (STEMCELL Technologies) by using gradient method with the Ficoll-Paque (GE Healthcare), and frozen for later use. Cryopreserved PBMCs were cultured in RPMI complete medium supplied with 30 U/ml of human recombinant IL-2 for three days, and the CD56⁺CD3⁻ NK cells were isolated by using the human NK cell isolation kit (Miltenyi Biotec) following the manufacturer’s instructions.

Zhou D., Park J.G., Wu Z., Huang H., Fiches G.N., Biswas A., Li T.W., Ma Q., Martinez-Sobrido L., Santoso N, & Zhu J. (2021). FACT subunit SUPT16H associates with BRD4 and contributes to silencing of antiviral interferon signaling. bioRxiv.

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