Fab fragment anti mouse igg

Manufactured by Jackson ImmunoResearch

Sourced in United States

The FAB fragment anti-mouse IgG is a laboratory tool used to detect and study mouse immunoglobulin G (IgG) proteins. It is derived from antibodies and specifically binds to the Fab region of mouse IgG, allowing for the identification and analysis of these molecules in various research applications.

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Lab products found in correlation

Donkey alexa 568 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions) Donkey serum, by Merck Group (2 mentions) Citrate buffer, by Agilent Technologies (1 mentions) Triton x 100, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Bovine serum albumin (bsa), by Jackson ImmunoResearch (1 mentions) S1699, by Agilent Technologies (1 mentions) D9542, by Merck Group (1 mentions) Click it plus edu kit, by Thermo Fisher Scientific (1 mentions) Fluoview fv1000 microscope, by Olympus (1 mentions) Mab1430, by R&D Systems (1 mentions) Cellsense imaging software, by Olympus (1 mentions) Bx41 microscope, by Olympus (1 mentions) Triton x, by Avantor (1 mentions)

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Fab fragments (6 citations)

5 protocols using fab fragment anti mouse igg

Immunofluorescence Staining of Cultured Cells and Muscle Tissue

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Following fixation (4% paraformaldehyde [PFA], 10 min), cultured cells were washed with PBS, permeabilized for 10 min in 0.1% Triton X-100 (Sigma), and blocked with a solution containing 5% BSA (Sigma) and 0.05% Triton X-100 for 1 h at room temperature. Cells were incubated with primary antibodies overnight at 4°C, and then with appropriate coupled secondary antibodies for 1 h at room temperature.
Muscles were dissected and snap frozen in liquid nitrogen-cooled isopentane. Muscle sections (10 μm) were fixed with 4% PFA for 20 min, permeabilized in cold methanol for 6 min, and boiled in citrate buffer (Dako) for epitope retrieval. The blocking was performed for 2 h at room temperature with BSA 5% and then for 30 min with anti-mouse IgG Fab fragment (Jackson Laboratories). A detailed list of the antibodies used is provided in Table S4.

Gattazzo F., Laurent B., Relaix F., Rouard H, & Didier N. (2020). Distinct Phases of Postnatal Skeletal Muscle Growth Govern the Progressive Establishment of Muscle Stem Cell Quiescence. Stem Cell Reports, 15(3), 597-611.

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Immunofluorescent Staining of Muscle Sections

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Cryosections of 10 µm were performed on TA muscles previously dissected and frozen in liquid-nitrogen-cooled isopentane 7 or 28 dpi. The muscle sections or the cultured cells were fixed in 4% PFA for 15 min at room temperature, permeabilized in cold methanol for 6 min, boiled in citrate buffer (Dako, S1699) for epitope retrieval and blocked with 5% immunoglobin G (IgG)-free BSA (Jackson, 001-000-162) for 1 h at room temperature. To reduce background, the slides were incubated 30 min at room temperature with anti-mouse IgG Fab fragment (Jackson, 115-007-003). The sections were incubated with primary antibodies (Supplementary Table 1) diluted in 5% IgG-free BSA overnight at 4 °C. Secondary antibody incubation (Invitrogen, Alexa-Fluor) was performed 1 h at room temperature followed by DAPI (Sigma, D9542) staining (1:5000) 10 min at room temperature. EdU staining reaction was performed according to manufacturer’s guidelines (Invitrogen, EdU Click-iT PLUS Kit, C10640).

Esteves de Lima J., Bou Akar R., Machado L., Li Y., Drayton-Libotte B., Dilworth F.J, & Relaix F. (2021). HIRA stabilizes skeletal muscle lineage identity. Nature Communications, 12, 3450.

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Immunofluorescent Staining of Plexin-A4

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CD8⁺ T cells and peripheral blood leukocytes were seeded onto glass slides by cytospin centrifugation and fixed in 4% formaldehyde for 10 minutes, followed by incubation with 0.2% Triton-X (VWR, 1.086.031.000) diluted in PBS for 15 minutes. To reduce the immune background, sections were blocked with 10% donkey serum (Sigma, D9663) in PBS for 1 hour, followed by blocking with FAB fragment anti-mouse IgG (Jackson Immunoresearch, 715–007–003, 1:10) for 1 hour. Samples were then probed overnight with mouse anti-Plexin-A4 (Plxna4; R&D Systems, 707201, 1:500) and incubated with Donkey Alexa 568–conjugated secondary antibodies (Molecular Probes, A10037, 1:100) for 45 minutes. Nuclei were counterstained with Hoechst-33342 and mounting of the slides was performed with ProLong Gold mounting medium without DAPI. All steps were performed at room temperature. Microscopy was conducted with an Olympus BX41 microscope and cellSens imaging software.

Celus W., Oliveira A.I., Rivis S., Van Acker H.H., Landeloos E., Serneels J., Cafarello S.T., Van Herck Y., Mastrantonio R., Köhler A., Garg A.D., Flamand V., Tamagnone L., Marine J.C., Matteo M.D., Costa B.M., Bechter O, & Mazzone M. (2021). Plexin-A4 Mediates Cytotoxic T-cell Trafficking and Exclusion in Cancer. Cancer Immunology Research, 10(1), 126-141.

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Immunofluorescence Staining of Mouse Brain

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Mouse brains were formalin-fixed and paraffin-embedded, and 4-µm sections were cut for hematoxylin and eosin (H&E) staining. For immunofluorescence, sequential staining was used. First, GBM tissue sections were stained with mouse anti-FGL2 antibody (1∶100, H00010875; Novus Biologicals, Littleton, CO, USA) overnight at 4 °C, followed by 1 h incubation with anti-mouse Alexa Fluor 647-conjugated antibody. The sections were blocked with Fab fragment anti-mouse IgG (15-007-003; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) for 1 h, and then stained with rabbit anti-CD31 antibody (bs-0468R, 1:100; Bioss Inc., Woburn, MA, USA), mouse anti-CD45 antibody (MAB1430, 10 µg/mL; R&D Systems), or Alexa Fluor 488-conjugated GFAP (53-9892, 5 μg/mL; Thermo Fisher Scientific) overnight at 4 °C, followed by incubation with Alexa Fluor 488-conjugated anti-mouse/rabbit antibody (1∶300; Invitrogen) for 1 h. ProLong Gold Antifade Mountant with 4′-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was used as the mounting medium. Slides were further processed for imaging analysis using an Olympus Fluoview FV1000 microscope.

Yan J., Zhao Q., Gabrusiewicz K., Kong L.Y., Xia X., Wang J., Ott M., Xu J., Davis R.E., Huo L., Rao G., Sun S.C., Watowich S.S., Heimberger A.B, & Li S. (2019). FGL2 promotes tumor progression in the CNS by suppressing CD103+ dendritic cell differentiation. Nature Communications, 10, 448.

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Immunohistochemical analysis of PlexinA4 expression

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CD8⁺ T cells and peripherical blood leukocytes were seeded onto glass slides by cytospin centrifugation and fixed in 4% formaldehyde for 10 minutes, followed by incubation with 0.2% Triton-X (VWR, 1.086.031.000) diluted in PBS for 15 minutes. To reduce the immune background, sections were blocked with 10% donkey serum (Sigma, D9663) in PBS for 1 hour, followed by blocking with FAB fragment anti-mouse IgG (Jackson Immunoresearch, 715-007-003, 1:10) for 1 hour. Samples were then probed overnight with mouse anti-PlexinA4 (R&D, 707201, 1:500) and incubated with Donkey Alexa 568-conjugated secondary antibodies (Molecular Probes, A10037, 1:100) for 45 minutes. Nuclei were counterstained with Hoechst-33342 and mounting of the slides was performed with ProLong Gold mounting medium without DAPI. All steps were performed at RT. Microscopy was conducted with an Olympus BX41 microscope and CellSense imaging software.

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