Serum was separated from trunk blood 24 h following the LPS injection by centrifugation at 4,000 × g for 5 min and was subsequently stored at −20°C until use. Serum corticosterone levels were measured using ELISA kits (Corticosterone ELISA kit; EIAab Science Co, Ltd., Wuhan, China), according to the manufacturer's instructions. The concentrations of interleukin (IL)-1β, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-6, IL-4, IL-10, brain-derived neurotrophic factor (BDNF) and insulin-like growth factor (IGF)-1 in several brain zones were determined using commercially available ELISA assays, according to the instructions supplied by the manufacturer (Mouse IL-1β ELISA set, Mouse IFN-γ (AN-18) ELISA set, Mouse IL-4 ELISA set, Mouse IL-10 ELISA set; all purchased from BD Pharmingen™; BD Biosciences, Franklin Lakes, NJ, USA Mouse TNF-α ELISA kit, Mouse IL-6 ELISA kit, Mouse BDNF ELISA kit, Mouse IGF-1 ELISA kit; all purchased from EIAab Science Co, Ltd.).
Mouse ifn γ an 18 elisa set
Manufactured by BD
Sourced in United States
The Mouse IFN-γ (AN-18) ELISA set is a laboratory equipment product designed for the quantitative measurement of interferon-gamma (IFN-γ) in mouse samples using the enzyme-linked immunosorbent assay (ELISA) technique. The set includes the necessary components to perform the ELISA assay, such as the capture and detection antibodies, standards, and reagents.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mouse ifn γ an 18 elisa set
1
Measurement of Serum and Brain Biomarkers
2
Serum Cytokine and Corticosterone Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After the collection of stool samples, the mice were anesthetized deeply with 10% chloral hydrate before the blood was collected from the heart. The blood samples stored in tubes were left at room temperature for 30 min and then centrifuged (4,000g, 10 min, at 4°C) for serum separation. Then, the prepared serum samples were used strictly according to the manufacturer’s protocols for ELISA assays to determine the levels of IFN-γ, TNF-α, IL-6, IL-4, IL-10, and corticosterone. Mouse IFN-γ (AN-18) ELISA set, Mouse IL-4 ELISA set, and Mouse IL-10 ELISA set were purchased from BD PharmingenTM (BD Biosciences). Mouse TNF-α ELISA kit, Mouse IL-6 ELISA kit, and corticosterone ELISA kit were purchased from EIAab Science Co, Ltd.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!