Anti myc m5546

Manufactured by Merck Group

Anti-Myc (M5546) is a mouse monoclonal antibody that recognizes the c-Myc epitope tag. It can be used for the detection and immunopurification of c-Myc-tagged recombinant proteins.

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Lab products found in correlation

Anti gapdh sc 47724, by Santa Cruz Biotechnology (2 mentions) Anti lps o11 b4, by Merck Group (2 mentions) Anti gsdmd sc 393581, by Santa Cruz Biotechnology (2 mentions) Cytotox 96 ldh release assay kit, by Promega (2 mentions) Ab6556, by Abcam (1 mentions) Horseradish peroxidase coupled secondary antibody, by Merck Group (1 mentions) A6455, by Thermo Fisher Scientific (1 mentions) Anti gst, by Rockland Immunochemicals (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Anti gapdh, by Merck Group (1 mentions) Western lightning plus ecl, by PerkinElmer (1 mentions) X omat, by Kodak (1 mentions) Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Nigericin, by InvivoGen (1 mentions) Pam3csk4 tlrl pms, by InvivoGen (1 mentions)

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Anti-Myc (274 citations) M5546 (10 citations)

4 protocols using anti myc m5546

Immunoblotting and Cytotoxicity Assay Reagents

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Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads, anti-Myc (M5546), and anti-LPS (O11:B4) were purchased from Sigma-Aldrich. Anti-GSDMD (sc-393581) and anti-GAPDH (sc-47724) were from Santa Cruz Biotechnology. Anti-Ub (3936), anti-HA (3724), anti-β-actin (3700), and the secondary antibodies used for immunoblotting and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901) was purchased from Abcam, whereas Nigericin, Pam3CSK4 (tlrl-pms) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.

Shi Y., Yang Y., Xu W., Shi D., Xu W., Fu X., Lv Q., Xia J, & Shi F. (2022). E3 ubiquitin ligase SYVN1 is a key positive regulator for GSDMD-mediated pyroptosis. Cell Death & Disease, 13(2), 106.

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Antibodies for Protein Localization and Detection

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Antibodies against Sec61 were as described (132 (link)). H. D. Schmitt provided anti-Emp47 antibodies. We derived monoclonal anti-HA antibodies from hybridoma cells (12CH5). We purchased anti-PGK (A6457, Molecular Probes), anti-GST (600-101-200, Rockland), polyclonal anti-GFP for immunoprecipitation (A6455, Molecular Probes), monoclonal anti-GFP for immunodetection (JL-8, Clontech), anti-Myc (M5546, Sigma–Aldrich), anti-FLAG (M2, F3165, Sigma–Aldrich), and anti-GAPDH (MAB374, Sigma–Aldrich). Horseradish peroxidase–coupled secondary antibodies (Sigma–Aldrich) were used to visualize immunoblots by Western Lightning Plus ECL (PerkinElmer Life Sciences) and autoradiography films (X-Omat, Kodak). For immunogold labelings, a rabbit anti-GFP antibody (Abcam, ab6556; diluted 1:100) and a 12-nm colloidal gold goat anti-rabbit secondary antibody (Dianova, 111-205-144; diluted 1:30) were used.

Meusser B., Purfuerst B, & Luft F.C. (2021). HIV-1 Gag release from yeast reveals ESCRT interaction with the Gag N-terminal protein region. The Journal of Biological Chemistry, 295(52), 17950-17972.

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Affinity purification of TDP-43 complexes

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MG132 was from Caymen Chemical. Di(N-succinimidyl) glutarate (DSG) was from Sigma-Aldrich. Anti-C-terminal TDP43 (12892-1-AP) was from Proteintech. Anti-BAG6 (sc-365928), anti-fibrillarin (sc-166021), and anti β-Actin (sc-47778) were from Santa Cruz Biotechnology. Anti-FLAG M2 (F1804), anti-FLAG M2 Magnetic Beads (M8823), and anti-Myc (M5546) was from Sigma-Aldrich. Secondary antibodies: anti-mouse IgG (H + L)-AlexaFluor488 (A-11001), anti-mouse IgG DyLight 680 (35519), anti-mouse IgG DyLight 800 (SA5-10176), anti-rabbit IgG DyLight 680 (35569), and anti-rabbit IgG DyLight 800 (SA5-10036) were from Thermo Scientific.

Kasu Y.A., Arva A., Johnson J., Sajan C., Manzano J., Hennes A., Haynes J, & Brower C.S. (2022). BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43. iScience, 25(5), 104273.

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Antibody Reagents for Protein Analysis

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Anti-Flag M2 (F1804), anti-Flag M2 Magnetic Beads, anti-Myc (M5546) and anti-LPS (O11:B4) were purchased from Sigma-Aldrich. Anti-GSDMD (sc-393581) and anti-GAPDH (sc-47724) were from Santa Cruz Biotechnology. Anti-Ub (sc-8017), anti-HA (3724), anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology.
Anti-SYVN1 (ab170901) was purchased from Abcam, whereas Nigericin, Pam3CSK4 (tlrl-pms) were purchased from InvivoGen. ELISA kits for analysis of human IL-1β were purchased from MultiSciences. CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.

Shi Y., Yang Y., Xu W., Xu W., Fu X., Lv Q., Xia J., & Shi F. (2021). E3 ubiquitin ligase SYVN1 is a key positive regulator for GSDMD-mediated pyroptosis.

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