Trace dsq single quadrupole mass spectrometer

A TRACE DSQ single quadrupole mass spectrometer from Thermo Fisher was used to perform the GC‐MS analyses, based on the way developed by Verslues (2017) with faint modifications. The GC conditions used were as follows: column: ZB‐5MS (Phenomenex), 0.25 µm film thickness, 30 mm × 0.25 mm; carrier gas: helium; split flow: 10 ml/min; linear velocity: injector temperature: 230°C; constant flow rate at 1.3 ml/min; and column temperature program: start temperature (40°C) held for 1 min, after which, increasing to 310°C at 5°C/min. There are MS conditions: ionization: detection: positive ion; electron impact (70 eV); full scan analyses: 10–600 m/z at the rate of two scans per second. Volatile metabolites were eluted by using the solvent front in this method, so GC separation of these analyses began with an initial start temperature at 40°C which was maintained for 2 min. The temperature was increased to 80°C at 10°C/min. The temperature was then kept constant at 80°C for 3 min before increasing to 230°C at a rate of 30°C/min.

The polyphenol complexes extracted from the three seaweed species under the optimized processing condition of each were subjected to gas chromatography–mass spectrometry (GC‐MS) analyses (Rahaman et al., 2019 (link), 2020 (link)). GC‐MS analyses were performed on a Thermo Fisher (San Jose, CA) TRACE DSQ single‐quadrupole mass spectrometer following the method developed by Verslues (2017 ) with slight modifications. The GC conditions were as follows: column, ZB‐5MS (Phenomenex; Torrance CA), 30 m × 0.25 mm, 0.25 µm film thickness; carrier gas, helium; linear velocity, 1.3 ml/min (constant flow); split flow, 10 ml/min; injector temperature, 230°C; column temperature program, and initial temperature of 40°C held for 1 min followed by an increase to 310°C at 5°C/min. The MS conditions were as follows: ionization, electron impact (70 eV); detection, positive ion; full‐scan analyses, 10 m/z–600 m/z at 2 scans/s. Volatile metabolites were eluted with the solvent front using this method, so GC separation of these analyses started with an initial temperature of 40°C held for 2 min, followed by an increase to 80°C at 10°C/min. The temperature was maintained for 3 min at 80°C after which it was increased to 230°C at a rate of 30°C/min.