BALF samples from mice, patients’ sera and supernatants were collected and stored at −80 °C until analyses. Plasma samples were diluted with PBS (10x) prior to ELISA. ELISA was performed according to the manufacturer’s instructions. Briefly, the plates were coated with capture antibody at 4 °C overnight. The coated plate was then blocked with assay diluent for 1h at room temperature (RT), followed by sample incubation for 2 h, and detection antibody for 2 h. Avidin-HRP and TMB substrate were employed for the detection of the signal. The reaction was terminated with the Stop solution. The plates were read for absorbance at 450 nm with 570nm as the referring wavelength within 30 min. ELISA kits were purchased from various merchants as listed below: mouse CC10/CC16 (MyBiosource.inc), Human Uteroglobin DuoSet Elisa, mouse IL-1beta and mouse TNF-alpha (R&D system), mouse PGE2 (Enzo Life Sciences).
Mouse il 1beta and mouse tnf alpha
Manufactured by R&D Systems
Mouse IL-1beta and mouse TNF-alpha are recombinant proteins produced in E. coli. They are commonly used in research applications as standards or controls.
Automatically generated - may contain errors
2 protocols using mouse il 1beta and mouse tnf alpha
1
Quantifying Inflammatory Mediators in BALF and Sera
2
Quantifying Inflammatory Mediators in BALF and Sera
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BALF samples from mice, patients’ sera and supernatants were collected and stored at −80 °C until analyses. Plasma samples were diluted with PBS (10x) prior to ELISA. ELISA was performed according to the manufacturer’s instructions. Briefly, the plates were coated with capture antibody at 4 °C overnight. The coated plate was then blocked with assay diluent for 1h at room temperature (RT), followed by sample incubation for 2 h, and detection antibody for 2 h. Avidin-HRP and TMB substrate were employed for the detection of the signal. The reaction was terminated with the Stop solution. The plates were read for absorbance at 450 nm with 570nm as the referring wavelength within 30 min. ELISA kits were purchased from various merchants as listed below: mouse CC10/CC16 (MyBiosource.inc), Human Uteroglobin DuoSet Elisa, mouse IL-1beta and mouse TNF-alpha (R&D system), mouse PGE2 (Enzo Life Sciences).
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