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Pphln1

Manufactured by Merck Group
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PPHLN1 is a laboratory equipment product from Merck Group. It is designed for use in scientific research and analysis. The core function of PPHLN1 is to provide a tool for researchers to perform specific tasks related to their work. No further details on the intended use or capabilities of the product can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Mphosph8, by Proteintech (2 mentions) Morc2, by Fortis Life Sciences (2 mentions) Setdb1, by Proteintech (2 mentions) Dcaf1, by Proteintech (2 mentions) Flag tag (flag), by Novus Biologicals (2 mentions) Line 1 orf1p, by Merck Group (2 mentions) Flag tag (flag), by Merck Group (2 mentions)

2 protocols using pphln1

1

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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2

Western Blot Analysis of Protein Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in PBS, counted, normalized for cell number, and lysed directly in 1x SDS-PAGE sample buffer. Samples were run on NuPage 4–12% Bis-Tris gels followed by blotting onto nitrocellulose membranes. Primary antibodies used: FAM208A (Atlas, HPA00875), MPHOSPH8 (Proteintech, 16796–1-AP), PPHLN1 (Sigma, HPA038902), SETDB1 (Proteintech 11231–1-AP), DCAF1 (Proteintech, 11612–1-AP), FLAG (Novus, NB600–345), FLAG (Sigma, F1804, used for IP), MORC2 (Bethyl Labs, A300–149A), LINE-1 ORF1p (Millipore, MABC1152),and HA (Biolegend, 901501).
Yurkovetskiy L., Guney M.H., Kim K., Goh S.L., McCauley S., Dauphin A., Diehl W, & Luban J. (2018). Primate immunodeficiency virus Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex. Nature microbiology, 3(12), 1354-1361.
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