7h10 adc agar medium

Manufactured by Merck Group

Sourced in United States

The 7H10-ADC agar medium is a solid growth medium used for the cultivation and isolation of mycobacteria, particularly Mycobacterium tuberculosis. It is formulated according to the Middlebrook 7H10 agar base and supplemented with albumin, dextrose, and catalase (ADC) to provide essential nutrients for the growth of mycobacteria.

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7h9 medium, by Merck Group (1 mentions)

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7H10 agar (2 citations)

2 protocols using 7h10 adc agar medium

Culturing M. smegmatis and Mutants

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The M. smegmatis mc² 155 strain and its mutants were propagated in liquid 7H9 medium (Sigma‐Aldrich, Burlington, MA, USA) with 10% ADC supplement (5% BSA, 0.85% NaCl and 2% dextrose) and 0.05% tyloxapol or 7H10‐ADC agar medium (Sigma‐Aldrich) at 37 °C. In both cases, the carbon source was enriched with 0.5% (vol/vol) glycerol. Hygromycin and kanamycin were added at final concentrations of 150 and 25 μg·mL⁻¹ respectively. Purine supplements (adenine, guanine and hypoxanthine) were prepared as 75 mm stocks in DMSO and were added to a final concentration of 200 μm when required.

Knejzlík Z., Doležal M., Herkommerová K., Clarova K., Klíma M., Dedola M., Zborníková E., Rejman D, & Pichová I. (2022). The mycobacterial guaB1 gene encodes a guanosine 5′‐monophosphate reductase with a cystathionine‐β‐synthase domain. The Febs Journal, 289(18), 5571-5598.

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Cultivating Mycobacterium smegmatis Strains

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The M. smegmatis mc 2 155 strain and its mutants were propagated in liquid 7H9 medium (Sigma) with 10% ADC supplement (5% BSA, 0.85% NaCl, 2% dextrose) and 0.05% tyloxapol or 7H10-ADC agar medium (Sigma) at 37 °C. In both cases, the carbon source was enriched with 0.5% (vol/vol) glycerol. Hygromycin and kanamycin were added at final concentrations of 150 and 25 g/ml, respectively. Purine supplements (adenine, guanine and hypoxanthine) were prepared as 75 mM stocks in DMSO and were added to a final concentration of 200 M when required.

Knejzlík Z., Doležal M., Herkommerová K., Clarová K., Klíma M., Dedola M., Zbornı́ková E., Rejman D., & Pichová I. (2021). The mycobacterial guaB1 gene encodes a guanosine 5’-monophosphate reductase with a cystathione-β-synthase domain.

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