Female B6.129P2(C)-Mecp2tm1.1Bird/J (Heterozygous Mecp2 knockout, Jax:003890) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and maintained in laboratory on a C57BL/6 background. The mice were maintained and bred in-house under standard 12-h light-dark cycle conditions. They were provided with standard rodent chow and sterilized tap water ad libitum. Male B6.129P2(C)-Mecp2tm1.1Bird/J mice (Hemizygote Mecp2 knockout, Mecp2-null mice) at 4-8 weeks of age were used for the experiments, and age-matched wild-type (WT) mice were used as controls. All experiments were approved by the Institutional Animal Care and Use Committee of Fudan University (No. 2019-289).
B6.129p2 c mecp2tm1 1bird j
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
The B6.129P2(C)-Mecp2tm1.1Bird/J is a lab mouse model with a targeted mutation in the Mecp2 gene. The Mecp2 gene is involved in the regulation of gene expression and is associated with Rett syndrome, a neurodevelopmental disorder. This mouse model is used in research to study the mechanisms and effects of Mecp2 gene mutations.
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17 protocols using b6.129p2 c mecp2tm1 1bird j
1
Mecp2 Knockout Mouse Model
2
Generation of Rett Syndrome Mouse Model
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Female heterozygous Mecp2+/− mice (Strain name: B6.129P2(C)‐Mecp2tm1.1Bird/J; Stock number 003890) from Jackson Lab were crossbred with wild‐type (WT) male C57BL/6 mice to produce the RTT model mice with the genotype Mecp2−/Y. The PCR protocol from Jackson Lab was used to identify the genotype. All experimental procedures in mice were conducted in accordance with the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals and were approved by the Georgia State University Institutional Animal Care and Use Committee.
3
Genotyping and Phenotyping of Mecp2 Mice
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Mecp2-null Mouse Model Tissue Extraction
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All animal procedures and husbandry were approved by Emory University’s Institutional Animal Care and Use Committee. The Mecp2-null mouse model, B6.129P2(C)-Mecp2tm1.1Bird/J (Cat: 003890 RRID: IMSR_JAX:003890), was purchased from The Jackson Laboratory at 5–6 weeks of age and housed within Emory University’s Division of Animal Resources for about 4 days before tissue collection.
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Mecp2 Knockout Mouse Breeding and Morphological Analysis
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MECP2-Null Mice: Modeling Rett Syndrome
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All experimental procedures were conducted in accordance with the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals and were approved by the Georgia State University Institutional Animal Care and Use Committee. Female heterozygous mice (genotype: MECP2−/+; strain name: B6.129P2(C)-MECP2tm1.1Bird/J; stock number 003890) from Jackson Lab were crossbred with male C57BL/6 mice (wild type (WT)) to produce the MECP2-null mice with the genotype MECP2−/Y. The PCR protocol from Jackson Lab was used to identify the genotype. All experiments were done in male mice because the MECP2−/Y males offer a completely MECP2-null condition that is not always available in MECP2−/+ females owing to uncontrolled X-chromosome inactivation.
7
Mecp2 Heterozygous Mice Protocol
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All animal experimental protocols were approved by the Harvard University and/or Syracuse University Institutional Animal Care and Use Committee and adhere to NIH guidelines. Mice were group housed at a maximum of five mice per cage on a 12/12 h light/dark cycle and were given food and water ad libitum. CD-1 timed pregnant female mice were purchased from Charles River. Female Mecp2 heterozygous mice were purchased from The Jackson Laboratory (B6.129P2(C)-Mecp2tm1.1Bird/J; RRID:IMSR_JAX:003890 ), and were maintained on a C57BL/6 background. Genotypes were determined by PCR on genomic DNA as follows: Mecp2 mutant mice, forward primer oIMR1436 5′- GGT AAA GAC CCA TGT GAC CC −3’; reverse primer oIMR1437 5′- TCC ACC TAG CCT GCC TGT AC −3’; reverse primer oIMR1438 5′- GGC TTG CCA CAT GAC AA-3′.
8
Developing Neurological Disease Models in Mice
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All experiments were approved by the Animal Care and Use Committee of Tokyo Women’s Medical University and performed according to institutional guidelines. Both sexes of C57BL/6 mice (P10-P50; Japan SLC Inc., Hamamatsu, Japan) and male MeCP2 knockout (KO) mice [B6.129P2(C)-MeCP2tm1.1Bird/J, Stock number; 003890, The Jackson Laboratory, Bar Harbor, ME] were used for experiments. The mice were housed under controlled temperature and humidity conditions, and had free access to food and water. Mice were reared under a 12:12 h light: dark cycle, except in dark-rearing experiments, during which mouse cages were placed in a light-tight box.
9
Mecp2 Knockout Mouse Experiments
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Experiments were conducted in accordance with the standards of the Canadian Council on Animal Care with the approval of the Office of Research Ethics at the University of Manitoba. All experiments were conducted in accordance with animal experimentation guidelines (University of Manitoba). Mecp2 knockout/null mice (B6.129P2(C)-Mecp2tm1.1Bird/J) (hereafter referred to as Mecp2tm1.1Bird y/− for the null mice) were purchased from the Jackson Laboratory (USA) along with their wild type (WT) counterparts. All experimental procedures outlined here were reviewed and approved (protocol number 12-031/1) by the University of Manitoba Bannatyne Campus Protocol Management and Review Committee.
10
Mecp2 Mutant Mice: Generation and Characterization
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Experiments were performed with approval from the Committee for DNA transformation and the Institutional Animal Care and Use Committee of Nihon University under the approved protocol numbers 2014DEN001 (16 June, 2014) and AP13D026-1 (26 June, 2015). All procedures were reviewed by the Committee in advance of conducting the experiments. Hemizygous Mecp2−/y mice were generated by crossbreeding heterozygous Mecp2 mutant females (B6.129P2(C)-Mecp2tm1.1Bird/J, Jackson Laboratory, Bar Harbor, ME, USA) with C57BL/6J WT males (Sankyo Labo Service, Tokyo, Japan). The mice were provided ad libitum access to food pellets and water at 24 ± 1 °C in a holding room with an alternating 12-h light/dark cycle (lights on at 07:00). Genotyping for pups was performed on postnatal day 7 by PCR following the protocols supplied by The Jackson Laboratory (forward primer: 5′-GGTAAAGACCCATGTGACCC-3′; reverse primer: 5′-TCCACCTAGCCTGCCTGTAC-3′). Male pups born to C57BL/6J wild-type females (WT) were used as control in all the experiments.
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