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3 protocols using bisphenol p

1

Quantification of Bisphenol Compounds

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Bisphenol A (≥99%), Bisphenol AF (≥97%), Bisphenol AP (≥99%), 4,4'‐bisphenol F (≥98%), bisphenol P (≥99%), bisphenol S (≥98%), bisphenol Z (≥98%) and the derivatization reagent, pyridine‐3‐sulfonyl chloride hydrochloride (≥98%), were purchased from Sigma‐Aldrich (Diegem, Belgium). Bisphenol B (≥98%) and bisphenol E (≥98%) were obtained from TCI (Zwijndrecht, Belgium). Chemical structures, octanol‐water partition coefficients (log Kow) and pKa values of the analyzed compounds are shown in Table 1. Individual stock solutions of each analyte (ca 1 mg mL–1) and a mixture of them were prepared in methanol. Working standard solutions were made by appropriate dilution in methanol and then stored in amber glass vials at –20 °C.
All organic solvents were HPLC or LC/MS grade and all other chemicals were analytical reagent grade. Ultrapure water was produced using a Milli‐Q Gradient purification system from Merck Millipore (Bedford, MA, USA). Formic acid (98–100%), hydrochloric acid (37%), sodium hydroxide and anhydrous sodium carbonate were purchased from Merck (Darmstadt, Germany).
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2

Quantification of Bisphenol Compounds

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Bisphenol A (≥99%), Bisphenol AF (≥99%), Bisphenol AP (≥99%), bisphenol B (≥98%), bisphenol BP (≥98%), bisphenol C (≥98%), bisphenol E (≥98%), bisphenol F (≥98%), bisphenol G (≥98%), bisphenol M (≥99%), bisphenol P (≥99%), bisphenol PH (≥99%), bisphenol S (≥98%), bisphenol TMC (≥97%), and bisphenol Z (≥99%) were purchased from Sigma-Aldrich. Stock standard solutions (100 mg/L) were individually prepared by dissolving standard compounds in acetonitrile. A stock standard mixture containing all the individual standards (100 mg/L) was also prepared and stored at −20 °C. Working solutions for calibrations were prepared by serial dilution of the stock standard mixture with acetonitrile (Sigma-Aldrich). Beta-glucuronidase from E. coli (200 units/mL) was purchased from La Roche AG group. Bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% of trimethylchlorosilane (TMCS) solution was used for BPs derivatization and was purchased from Sigma-Aldrich. Ammonium acetate (98%), calcium chloride (CaCl2) (≥99%), creatinine hydrochloride (≥97%), ammonium phosphate dibasic (≥99%), magnesium chloride hexahydrate (≥99%), potassium chloride (≥99%), sodium sulphate (≥99%), urea (≥98%), acetone (99.9%), formic acid (≥95%), and methanol (99.9%) were purchased from Sigma-Aldrich. Nanopure water was provided by an ultrapure water system (ariumPro, Sartorius, Germany).
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3

Comprehensive Bisphenol Compound Acquisition

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Bisphenol S (BPS) (purity ≥ 98%), Bisphenol A (BPA) (purity ≥ 99%), Bisphenol E (BPE) (purity ≥ 98%), 2,2-Bis(4-hydroxy-3-methylphenyl)propane (3-3BPA) (purity ≥ 97%), Bisphenol B (BPB) (purity ≥ 98%), Bis(4-hydroxyphenyl)-2,2-dichloroethylene (BPC) (purity ≥ 98%), Bisphenol BP (BPBP)(purity ≥ 98%), Bisphenol F (BPF) (purity ≥ 98%), Bisphenol FL (BPFL) (purity ≥ 97%), Bisphenol Z (BPZ) (purity ≥ 98%), 4,4′-Dihydroxybiphenyl (BP4-4) (purity ≥ 97%), Bisphenol AP (BPAP) (purity ≥ 99%), Bisphenol AF (BPAF) (purity ≥ 97%), Bisphenol P (BPP) (purity ≥ 98%), Bisphenol M (BPM) (purity ≥ 99%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
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