Survival surgery was performed at 5~6 days before clamp experiments to establish an indwelling catheter in the jugular vein. On the day of the clamp experiment, mice were fasted overnight (~15 hrs), and a 2-hr hyperinsulinemic-euglycemic clamp was conducted in conscious mice with a primed and continuous infusion of human insulin (150 mU/kg body weight priming followed by 2.5 mU/kg/min; Humulin, Eli Lilly & Company, Indianapolis, IN, USA). To maintain euglycemia, 20% glucose was infused at variable rates during clamps. Whole body glucose turnover was assessed with a continuous infusion of [3-3H]glucose (PerkinElmer, Waltham, MA, USA), and 2-7deoxy-D-[1-14C]glucose (2-[14C]DG) was administered as a bolus (10 µCi) at 75 min after the start of clamps to measure insulin-stimulated glucose uptake in individual organs.
2 7deoxy d 1 14c glucose 2 14c dg
Manufactured by PerkinElmer
Sourced in United States
2-7deoxy-D-[1-14C]glucose (2-[14C]DG) is a radiolabeled glucose analog. It is used as a tool for metabolic studies and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 2 7deoxy d 1 14c glucose 2 14c dg
1
Hyperinsulinemic-Euglycemic Clamp in Mice
2
Hyperinsulinemic-Euglycemic Clamp in Mice
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Survival surgery was performed at 5~6 days before clamp experiments to establish an indwelling catheter in the jugular vein. On the day of the clamp experiment, mice were fasted overnight (~15 hrs), and a 2-hr hyperinsulinemic-euglycemic clamp was conducted in conscious mice with a primed and continuous infusion of human insulin (150 mU/kg body weight priming followed by 2.5 mU/kg/min; Humulin, Eli Lilly & Company, Indianapolis, IN, USA). To maintain euglycemia, 20% glucose was infused at variable rates during clamps. Whole body glucose turnover was assessed with a continuous infusion of [3-3H]glucose (PerkinElmer, Waltham, MA, USA), and 2-7deoxy-D-[1-14C]glucose (2-[14C]DG) was administered as a bolus (10 µCi) at 75 min after the start of clamps to measure insulin-stimulated glucose uptake in individual organs.
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