In this investigation, we followed the methods of chemical binding to proteins from our previous publication, in which we ascertained elevated levels of antibodies against xenobiotics in a subgroup of healthy subjects [19 (link)]. In that study, we identified antibodies to the same chemical haptens in about 20% of tested individuals. For this study, we used the same binding methods.
We purchased the HSA, BSA, hemoglobin, formaldehyde, tolylene-2.4-diisocyanate, trimellitic anhydride, p-amino benzoic acid, bisphenol-A (BPA), tetrabromobisphenol-A (TBBPA), isopropyl benzoic acid, cyanoethyl benzoic acid, propyl 4-hydroxybenzoic acid, permethrin, mercury chloride, nickel sulfate, cobalt acetate, cadmium chloride, lead acetate, and arsenic oxide from Sigma Aldrich (St. Louis, MO, USA).
We purchased the HSA, BSA, hemoglobin, formaldehyde, tolylene-2.4-diisocyanate, trimellitic anhydride, p-amino benzoic acid, bisphenol-A (BPA), tetrabromobisphenol-A (TBBPA), isopropyl benzoic acid, cyanoethyl benzoic acid, propyl 4-hydroxybenzoic acid, permethrin, mercury chloride, nickel sulfate, cobalt acetate, cadmium chloride, lead acetate, and arsenic oxide from Sigma Aldrich (St. Louis, MO, USA).