Propofol 2 6 diisopropylphenol

R-mTFD-MPAB was a gift from Prof. Karol Bruzik, PhD (Dept of Medicinal Chemistry and Pharmacognosy, University of Illinois, Chicago, USA). It was stored as a 100 mM solution in DMSO and diluted in electrophysiology buffer to 4, 8, or 16 µM for experiments. Alphaxalone was purchased from Tocris Bioscience (Bristol, UK), stored as a 10 mM solution in DMSO, and diluted to 1.25, 2.5, or 5 µM in electrophysiology buffer for experiments. Propofol (2,6-diisopropylphenol) was purchased from Sigma-Aldrich (St. Louis, MO, USA), stored as a 10 mM solution in DMSO and diluted to 2.5, 5, or 10 µM in electrophysiology buffer for experiments. R-Etomidate was purchased from Hospira, Inc (Lake Forest, IL, USA) as a 2 mg/ml (~8.2 mM) solution in 35% propylene glycol:water and diluted to 1.6, 3.2, or 6.4 µM in electrophysiology buffer for experiments. We have previously shown that DMSO and propylene glycol at the dilutions used here produce no effects on GABA_A receptor function ^{23 (link)}. Para-cloromercuribenzenesulfonic acid sodium salt (pCMBS) was purchased from Toronto Chemical Research (Toronto, ON, Canada) and fresh stock solutions in electrophysiology buffer were prepared on the day of use, and kept on ice until final dilution. γ-Aminobutyric acid (GABA), picrotoxin, salts, and buffers were purchased from Sigma-Aldrich.