Imidazole ketone erastin

Igf2bp3^−/− mice were generated by Cyagen Biosciences (Guangzhou, China). Mettl3^−/− mice were obtained as described in our previous study [25 (link)]. H1299 cells with or without IGF2BP3 overexpression were digested and adjusted to a density of 5 ​× ​10⁶ ​cells/200 ​μL. Next, 200 ​μL of cells were injected into the right armpit of each 4–6-week-old athymic nude mouse (Jiesijie, Shanghai, China). The weight and tumor size of nude mice were measured. Each group contained five mice. After 2 weeks, mice were injected daily with dimethyl sulfoxide (DMSO, Beyotime Biotechnology, Shanghai, China) with or without imidazole ketone erastin (IKE, 50 ​mg/kg, MedChemExpress, Monmouth, NJ, USA) or rigosertib (RIG, 250 ​mg/kg, Selleck, Houston, TX, USA). Mice were euthanized 28 ​d after implantation. Tumor growth was monitored and sizes were calculated as follows: 0.5 ​× ​L ​× ​W², where L indicates length and W indicates width. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital.

Normal C57BL/6 mice were purchased from the Institute of Zoology (Chinese Academy of Sciences, Beijing, China). B6.129P2-apoE^tm1Unc/J (ApoE^−/−; C57BL/6 background) and B6; 129S7-Ldlr^tm1Her/J (Ldlr^−/−; C57BL/6 background) mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). GFP-LC3 mice were kindly gifted by Dengqun Liu (University of Electronic Science and Technology of China). To induce hypercholesterolemia, normal C57BL/6 mice were fed a high-cholesterol diet (HCD) (D12108C, Research Diets, New Brunswick, NJ, USA) for 3 months. For rapamycin and imidazole ketone erastin (IKE) administration, mice were treated with a dose of 4 mg/kg rapamycin (MedChem Express, Monmouth Junction, NJ, USA) or 40 mg/kg imidazole ketone erastin (IKE, MedChem Express) body weight by intraperitoneal injection every other day for 1 month from the third month of HCD treatment. Mice were fed with autoclaved food and housed in specific pathogen-free conditions. All mice used were male, background-matched and age-matched (8–10 weeks of age). All animal procedures were performed in accordance with National Institutes of Health guidelines [28 ] and were approved by the Animal Care Committee of the Army Medical University (AMUWE2019092).