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Rabbit anti phospho erk p42 p44

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Rabbit anti-phospho-ERK p42/p44 is a primary antibody that specifically recognizes the phosphorylated form of extracellular signal-regulated kinase (ERK) p42 and p44. It can be used to detect the activation of the ERK signaling pathway.

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Lab products found in correlation

Rabbit anti ha, by Merck Group (2 mentions) Rabbit anti ulk1, by Merck Group (2 mentions) Nupage 4 12 bis tris precast gel, by Thermo Fisher Scientific (2 mentions) Rabbit anti akt, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho akt t308, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho akt s473, by Thermo Fisher Scientific (2 mentions) Rabbit anti phospho sgk1 ser78, by Thermo Fisher Scientific (2 mentions) Anti phospho sgk1 thr256, by Thermo Fisher Scientific (2 mentions) Rabbit anti cx37, by Abcam (2 mentions) Rabbit anti lc3a b, by Abcam (2 mentions) Rabbit anti atg101, by Merck Group (2 mentions) Rabbit anti rb1cc1, by Merck Group (2 mentions) Rabbit anti atg13, by Merck Group (2 mentions) Mouse anti erk, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

Anti-phospho ERK p42/p44 Phospho-p42/p44 ERK Anti-ERK (p44/p42, ERK p44/p42 Rabbit phospho-ERK Anti-phospho-ERK Rabbit anti-ERK Phospho-ERK ERK/phospho-ERK Anti-ERK

2 protocols using rabbit anti phospho erk p42 p44

1

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated from passage 5 HUVECs 18 h after induction of gene expression in 1% SDS lysis buffer containing protease and phosphatase inhibitor. 10 or 20 μg of protein was run on a NuPAGE 4%–12% precast Bis-Tris gel (Invitrogen) and transferred onto nitrocellulose membranes using a semi-dry (Bio-Rad) or a wet transfer method. Anti-human antibodies used are as follows: rabbit anti-phospho-ERK p42/p44 (Cell Signaling Technologies [CST] 9106) 1:1,000, mouse anti-ERK (CST 9102) 1:1,000, rabbit anti-Akt (CST 9272) 1:1,000, rabbit anti-phospho-Akt-T308 (CST 4056), rabbit anti-phospho-Akt-S473 (CST 4051), rabbit anti-serum/glucocorticoid-regulated kinase 1 (SGK1) (CST 12103) 1:1,000, rabbit anti-phospho-SGK1-Ser78 (CST 5599) 1:1,000, anti-phospho-SGK1-Thr256 (Thermo Fisher 44–1260G) 1:1,000, rabbit anti-Cx37 (Abcam 181701) 1:3,000, rabbit anti-LC3A/B (CST 4108) 1:1,000, rabbit anti-ULK1 (CST 8054) 1:1,000, rabbit anti-Atg13 (CST 13273) 1:1,000, rabbit anti-Atg101 (CST 13492) 1:1,000, rabbit anti-RB1CC1 (CST 12436) 1:1,000, rabbit anti-HA (CST 3724) 1:1,000, Actin (Sigma Aldrich A1978) 1:4,000. The following secondary antibodies were used: anti-mouse HRP-linked antibody (CST 7076) 1:2,500 or anti-rabbit HRP-linked antibody (CST 7074) 1:2,500. Quantification of western blots was performed using ImageJ (v1.51).
Ugwu N., Atzmony L., Ellis K.T., Panse G., Jain D., Ko C.J., Nassiri N, & Choate K.A. (2021). Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation. Human Genetics and Genomics Advances, 2(2), 100028.
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2

Western Blot Analysis of Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein was isolated from passage 5 HUVECs 18 h after induction of gene expression in 1% SDS lysis buffer containing protease and phosphatase inhibitor. 10 or 20 μg of protein was run on a NuPAGE 4%–12% precast Bis-Tris gel (Invitrogen) and transferred onto nitrocellulose membranes using a semi-dry (Bio-Rad) or a wet transfer method. Anti-human antibodies used are as follows: rabbit anti-phospho-ERK p42/p44 (Cell Signaling Technologies [CST] 9106) 1:1,000, mouse anti-ERK (CST 9102) 1:1,000, rabbit anti-Akt (CST 9272) 1:1,000, rabbit anti-phospho-Akt-T308 (CST 4056), rabbit anti-phospho-Akt-S473 (CST 4051), rabbit anti-serum/glucocorticoid-regulated kinase 1 (SGK1) (CST 12103) 1:1,000, rabbit anti-phospho-SGK1-Ser78 (CST 5599) 1:1,000, anti-phospho-SGK1-Thr256 (Thermo Fisher 44–1260G) 1:1,000, rabbit anti-Cx37 (Abcam 181701) 1:3,000, rabbit anti-LC3A/B (CST 4108) 1:1,000, rabbit anti-ULK1 (CST 8054) 1:1,000, rabbit anti-Atg13 (CST 13273) 1:1,000, rabbit anti-Atg101 (CST 13492) 1:1,000, rabbit anti-RB1CC1 (CST 12436) 1:1,000, rabbit anti-HA (CST 3724) 1:1,000, Actin (Sigma Aldrich A1978) 1:4,000. The following secondary antibodies were used: anti-mouse HRP-linked antibody (CST 7076) 1:2,500 or anti-rabbit HRP-linked antibody (CST 7074) 1:2,500. Quantification of western blots was performed using ImageJ (v1.51).
Ugwu N., Atzmony L., Ellis K.T., Panse G., Jain D., Ko C.J., Nassiri N, & Choate K.A. (2021). Cutaneous and hepatic vascular lesions due to a recurrent somatic GJA4 mutation reveal a pathway for vascular malformation. Human Genetics and Genomics Advances, 2(2), 100028.
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