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Anti hsv 1 icp0

Manufactured by Santa Cruz Biotechnology
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Anti-HSV-1 ICP0 is a laboratory reagent used for the detection and analysis of the Herpes Simplex Virus type 1 (HSV-1) Infected Cell Protein 0 (ICP0). It is a critical regulator of viral gene expression and replication.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti actin, by Merck Group (1 mentions) Anti myc, by Merck Group (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Anti flag m2 hrp, by Merck Group (1 mentions) Anti mlkl phospho s358, by Abcam (1 mentions) Anti mlkl, by Abcam (1 mentions) Z vad fmk, by Santa Cruz Biotechnology (1 mentions)

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Anti-ICP0 (8 citations)

2 protocols using anti hsv 1 icp0

1

Indirect Immunofluorescence for HCMV and HSV-1

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Primary monoclonal antibodies—anti-HCMV immediate early (IE) 1 (Mab63-27) [34 (link)], anti-HCMV tegument protein pp65 (Mab28-77; kindly provided by W. Britt, Univ. Birmingham, AL, USA), and anti-HSV-1 ICP0 (11060, SantaCruz Biotech, Dallas, TX, USA)—were used together with secondary goat anti-mouse antibody Alexa Fluor® 488 (Invitrogen, Waltham, MA, USA) to detect infected cells in indirect immunofluorescence staining as described before [28 (link)].
Brenner S., Braun B., Read C., Weil T., Walther P., Schrader T., Münch J, & von Einem J. (2021). The Molecular Tweezer CLR01 Inhibits Antibody-Resistant Cell-to-Cell Spread of Human Cytomegalovirus. Viruses, 13(9), 1685.
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2

Recombinant TNF-α and Smac Mimetic Induce Necroptosis

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Recombinant human TNF-α (Invivogen, rhtnf-a), Smac mimetic RMT5265 (also referred to as SMAC007) (Li et al., 2004 (link)), and zVAD (Z-VAD-FMK, Santa Cruz, sc-311561) (TSZ) were used at 10 ng/mL, 10 nM, and 20 μM, respectively. HSV-1 infections were performed at MOI = 5. Doxycycline (DOX) was used between 100 ng/mL and 500 ng/mL, as specified for each experiment. The RIPK3 inhibitor GSK840 (GlaxoSmithKline) was used at 3 μM and the MLKL inhibitor NSA (Calbiochem, #480073) was used at 0.2 μM. The following antibodies were used: Anti-FLAG M2-HRP (Sigma, A8592), Anti-MLKL (Abcam, ab184718), Anti-MLKL phospho S358 (Abcam, ab187091), Anti-Myc (Sigma, M4439), Anti-ITPK1 (GeneTex, GTX107974), Anti-HSV-1 ICP0 (Santa Cruz, 11060), Anti-GAPDH (Santa Cruz, sc-32233), Anti-IMMT (Protein Tech, 10179-1-AP), and Anti-Actin (Sigma, A2066).
Dovey C.M., Diep J., Clarke B.P., Hale A.T., McNamara D.E., Guo H., Brown NW J.r., Cao J.Y., Grace C.R., Gough P.J., Bertin J., Dixon S.J., Fiedler D., Mocarski E.S., Kaiser W.J., Moldoveanu T., York J.D, & Carette J.E. (2018). MLKL requires the inositol phosphate code to execute necroptosis. Molecular cell, 70(5), 936-948.e7.
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