Human claudin 5 elisa kit

Serum samples were taken immediately upon admission at the emergency department before rt-PA administration or endovascular therapy and stored at < -80°C. Serum biomarker levels were determined using commercially available quantitative ELISA kits as follows: MMP-9, human ELISA kits for MMP-9 (PK-EL-64106, PromoCell, Heidelberg, Germany), amyloid precursor protein (APP) 770, Human ELISA kit of APP770 (#27736, Immuno-Biological Laboratories, Fujioka, Japan); endothelin-1, endothelin-1 ELISA kit (ADI-900-020A, EnzoLifescience, Lausen, Switzerland); S100B, Human ELISA kit of S100B (DY1820-05, DRG MedTek, Warsaw, Poland); and claudin-5, human claudin-5 ELISA kit (NBP2-75332, Novus Biologicals, CO, USA).

Serum was split into 500 μL aliquots to measure BDNF (R&D Systems, Minneapolis, MN, USA), zonulin (Human Zonulin ELISA Kit, Elabscience, Bethesda, MD, USA), lipopolysaccharide (LPS, LSBio, Seattle, WA, USA), Fatty Acid Binding Protein 2 (FABP2, Human FABP2 Quantikine ELISA Kit, R&D Systems, Minneapolis, MN, USA), and claudin-5 (Human Claudin-5 ELISA Kit, Novus Biologicals, Littleton, CO, USA).