Anti cd45.1 clone a20

Manufactured by BD

Anti-CD45.1 (clone A20) is a monoclonal antibody that targets the mouse CD45.1 antigen. CD45 is a transmembrane protein tyrosine phosphatase expressed on the surface of most hematopoietic cells. The A20 clone is specific for the CD45.1 allelic variant.

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Lab products found in correlation

Anti cd28 37, by BioLegend (1 mentions) Anti α tubulin dm1a, by Thermo Fisher Scientific (1 mentions) Anti p53 clone 1c12, by Cell Signaling Technology (1 mentions) Anti cd3ε 145 2c11, by BioLegend (1 mentions) Collagenase type 2, by Worthington (1 mentions) Anti cd45.2 clone 104, by Thermo Fisher Scientific (1 mentions) Anti cd64 clone x54 5 7.1, by BioLegend (1 mentions) Anti cd31 clone mec13, by BD (1 mentions) Dnase, by Merck Group (1 mentions) Anti cd11b clone m1 70, by BioLegend (1 mentions) Anti ly6g clone 1a8, by BioLegend (1 mentions) Anti b220 clone ra3 6b2, by Thermo Fisher Scientific (1 mentions) Anti ter119 clone ter119, by Thermo Fisher Scientific (1 mentions) Anti sca 1 clone d7, by BioLegend (1 mentions) Anti cd19 clone 1d3, by BD (1 mentions) Live dead fixable violet dead cell dye, by Thermo Fisher Scientific (1 mentions) Anti cd45.2 clone 104, by BD (1 mentions) Anti cd115 clone afs98, by Thermo Fisher Scientific (1 mentions) Anti cd34 clone ram34, by BD (1 mentions) Anti cd3e clone 145 2c11, by BioLegend (1 mentions)

Close spelling variants of this product

Anti-CD45 (213 citations) CD45.1 (A20; (13 citations) CD45.1 (Clone A20) (10 citations) Anti-CD45.1 (A20; (7 citations) Anti-CD45.1-PE (clone A20; (2 citations)

3 protocols using anti cd45.1 clone a20

Antibody Panel for T Cell Studies

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The following antibodies were used.
For stimulation and cell culture: anti-CD3ε (145-2C11) and anti-CD28 (37.51) antibodies were from BioLegend. For cell sorting and flow cytometry analysis: anti-CD8α (clone 53-6.7), anti-CD4 (clone RM4-5), anti-CD24 (clone M1/69), anti-TCRβ (clone H57-597), anti-Vα11 (clone RR8-1), anti-CD5 (clone 53-7.3), anti-CD69 (clone H1.2F3), anti-B220 (clone RA3-6B2), anti-Gr1 (clone RB6-8C5), anti-CD11b (clone M1/70), anti-CD27 (clone LG.3A10), anti-CD11c (clone N418), anti-Ter119 (clone TER119), anti-CD3 (clone 145-2C11), anti-NK1.1 (clone PK136), anti-TCRγδ (clone GL3), anti-CD44 (clone IM7), anti-CD25 (clone PC61.5), anti-CD71 (clone R17217), anti-IL-7R (clone A7R34), anti-BCL-2 (clone 3F11), anti-CD19 (clone 1D3/CD19), anti-c-kit (clone 2B8), anti-IgM (clone RMM-1), and anti-CD45.1 (clone A20) were from BD Biosciences and BioLegend. For imaging studies: anti-γ-tubulin (clone 14C11) was from BioLegend and anti-α-tubulin (DM1A) was from (Thermo Fisher Scientific). For immunoprecipitation and Western blot analysis: anti-DIC (clone 74-1), IgG2b isotype control (sc-3879), anti-LIS1 (sc-15319), and anti-DHC (sc-9115) were from Santa Cruz Biotechnologies. Anti-p150glued (clone 1/p150Glued) were from BD Biosciences, anti-p53 (clone 1C12) were from Cell Signaling, and anti-Rac1 (clone 23A8) were from Millipore.

Argenty J., Rouquié N., Bories C., Mélique S., Duplan-Eche V., Saoudi A., Fazilleau N, & Lesourne R. (2022). A selective LIS1 requirement for mitotic spindle assembly discriminates distinct T-cell division mechanisms within the T-cell lineage. eLife, 11, e80277.

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Isolation and Characterization of Cardiac Cells

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Heart tissue was cut into small pieces and digested by placement into a RPMI 1640 solution containing Type 2 collagenase (1 mg/ml) (Worthington Biochemical Corporation) and 10 U/ml DNase (Sigma-Aldrich) at 37°C for 60 minutes. The digested tissue was then passed through a 70-μm cell strainer and treated with ACK lysing buffer. Cells were stained with fluorochrome-labeled anti-CD45.2 (clone 104, eBioscience), anti-CD45.1 (clone A20, BD Biosciences), anti-MHC class II (clone M5/114.15.2, BioLegend), anti CD11b (clone M1/70, BioLegend), anti-CCR2 (clone 575301, R&D Systems), anti-CD64 (clone X54-5/7.1, BioLegend), and anti-CD31 (clone MEC13.3, BD Biosciences).

Li W., Hsiao H.M., Higashikubo R., Saunders B.T., Bharat A., Goldstein D.R., Krupnick A.S., Gelman A.E., Lavine K.J, & Kreisel D. (2016). Heart-resident CCR2+ macrophages promote neutrophil extravasation through TLR9/MyD88/CXCL5 signaling. JCI Insight, 1(12), e87315.

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Detailed FACS Sorting and Analysis

Cited 1 time

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For FACS sorting and analysis, we used previously described staining protocols (Mossadegh-Keller et al., 2013 (link)), published stem and progenitor cell definitions (Bryder et al., 2006 (link)), FACSCanto, LSRII, and FACSAriaIII equipment, and DIVA software (BD), analyzing only populations with at least 200 events. The following antibodies were used for staining cells: anti-CD117 (clone 2B8; BD), anti–Sca-1 (clone D7; BioLegend), anti-CD34 (clone RAM34; BD), anti-CD16/32 (clone 2.4G2; BD), anti-CD11b (clone M1/70; BD), anti-CD19 (clone 1D3; BD), anti-CD3e (clone 145-2C11; BioLegend), anti-Ly6G (clone 1A8; BioLegend), anti-Ly6C (clone HK1.4; BioLegend), anti-CD115 (clone AFS98; eBioscience), anti-CD45.2 (clone 104; BD), anti-CD45.1 (clone A20; BD), anti-B220 (clone RA3-6B2; eBioscience), anti-Ter119 (clone TER-119; eBioscience), and anti-CD71 (clone R17217; eBioscience). LIVE/DEAD Fixable Violet Dead cell dye (Invitrogen) was used as a viability marker. Information describing gating strategies is shown in Figs. S1 and S2.

Kandalla P.K., Sarrazin S., Molawi K., Berruyer C., Redelberger D., Favel A., Bordi C., de Bentzmann S, & Sieweke M.H. (2016). M-CSF improves protection against bacterial and fungal infections after hematopoietic stem/progenitor cell transplantation. The Journal of Experimental Medicine, 213(11), 2269-2279.

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