Plan apochromat 20 0.8 air objective

Manufactured by Zeiss

The Plan-Apochromat 20×/0.8 air objective is a high-performance microscope objective lens manufactured by Zeiss. It features a magnification of 20x and a numerical aperture of 0.8, designed for use with air samples. The lens is optimized for delivering high-quality, distortion-free images across a wide field of view.

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4 protocols using plan apochromat 20 0.8 air objective

Whole-mount Immunohistochemistry of Zebrafish

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For whole-mount immunohistochemistry, zebrafish embryos were deyolked and mounted onto glass slides with 70% glycerol. For synapse quantifications, images were acquired using a Zeiss LSM880 with Airyscan, Axio examiner confocal microscope with Plan-Apochromat 20×/0.8 air objective.
To image abnormal motor axons in response to various combinations of drug treatment, a Zeiss AxioImager Z1, with ApoTome.2 upright microscope was used.

Opris¸oreanu A.M., Smith H.L., Krix S., Chaytow H., Carragher N.O., Gillingwater T.H., Becker C.G, & Becker T. (2021). Automated in vivo drug screen in zebrafish identifies synapse-stabilising drugs with relevance to spinal muscular atrophy. Disease Models & Mechanisms, 14(4), dmm047761.

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Visualizing CXCR4+ Cells in Ischemic Hearts

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For immunofluorescence analysis, hearts were harvested 3 days after arterial ligation and were immediately embedded in OCT and frozen at − 80 °C. Frozen samples were cut with a cryotome (Leica CM1510, Nussloch, Germany) into 10 μm sections, fixed with 4% formaldehyde, and blocked with goat serum. The sections were incubated with monoclonal PE-conjugated anti mouse CXCR4 (clone2B11, eBioscience) or respective PE-labeled isotype control (Rat IgG2b kappa, eBioscience) for 1 hour at room temperature. DNA was stained with 1 µg/mL DAPI (Sigma), and a coverslip was placed using mounting medium (DAKO). Primary antibodies were applied 1:100 (final dilution). Images were acquired using a LSM 880 confocal microscope with Airyscan module and Plan-Apochromat 20×/0.8 air objective (Carl Zeiss Microscopy) and processed using ZEN software (Zeiss).

Zacherl M.J., Todica A., Wängler C., Schirrmacher R., Hajebrahimi M.A., Pircher J., Li X., Lindner S., Brendel M., Bartenstein P., Massberg S., Brunner S., Lehner S., Hacker M, & Huber B.C. (2020). Molecular imaging of cardiac CXCR4 expression in a mouse model of acute myocardial infarction using a novel 68Ga-mCXCL12 PET tracer. Journal of Nuclear Cardiology, 28(6), 2965-2975.

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Imaging of Organoids Using Confocal Microscopy

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Images were acquired with a Zeiss Axioplan2 using a Plan-Apochromat 20×/0.8 air objective or Zeiss LSM 710 laser scanning confocal microscope using Plan-Apochromat 20×/0.75 air and Plan-Apochromat 63×/1.40 oil objectives. Tiled and stitched images of sagittal sections were collected using a Plan-Apochromat 20×/0.75 air objective, controlled by Zen software (Zeiss).
Organoids were imaged using Cell Insight CX7-LZR (Thermo Scientific), and images were processed with HCS Studio Cell Analysis software (Thermo Scientific). Maximal projection z-stacks are presented, and co-localization was interpreted only in single z-stacks. z-stacks were projected using ImageJ. Images were assembled in Adobe Illustrator CC2015.3.

Konieczny P., Xing Y., Sidhu I., Subudhi I., Mansfield K.P., Hsieh B., Biancur D.E., Larsen S.B., Cammer M., Li D., Landén N.X., Loomis C., Heguy A., Tikhonova A.N., Tsirigos A, & Naik S. (2022). Interleukin-17 governs hypoxic adaptation of injured epithelium. Science (New York, N.Y.), 377(6602), eabg9302.

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Multimodal Imaging of Tissue Sections

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Images of tissue sections stained by immunofluorescence, Click-EdU labeling, and TUNEL staining were acquired by using the Zen 2 software (Zeiss) on an AxioImager M2 microscope (Zeiss) equipped with a high-resolution camera (pco.edge; sCMOs Cameras) and with either a Plan Apochromat 20×/0.8 air objective (420651-9911; Zeiss) or with a 40× Plan Apochromat 40×/0.95 air objective (420661-9970; Zeiss). Single-channel images were merged to create composite multichannel images with ImageJ software.
Images of Oil Red O– and toluidine-stained tissue sections were acquired by using AxioVision 4.8 software (Zeiss) on a Zeiss Axioskop 2 microscope equipped with an AxioCam HRc camera (Zeiss) and with either a Plan-Neofluar 20×/0.5 air objective (440341-9904; Zeiss), a Plan-Neofluar 40×/1.30 oil immersion objective (1022-818; Zeiss), or a Plan-Neofluar 100×/1.30 oil immersion objective (440480; Zeiss).
Images of nodal stainings were acquired on an LSM 780-FCS laser scanning confocal microscope (Zeiss) equipped with a Plan-Apochromat DIC 63×/1.4 oil immersion objective (421782-9900-799; Zeiss) using an argon laser (488 nm), a solid-state laser (561 nm), and a helium-neon laser (633 nm). For signal detection two Quasar photomultipliers and 32 photomultiplier GaAsP detectors were used.
All images were acquired at room temperature (20–24°C).

Montani L., Pereira J.A., Norrmén C., Pohl H.B., Tinelli E., Trötzmüller M., Figlia G., Dimas P., von Niederhäusern B., Schwager R., Jessberger S., Semenkovich C.F., Köfeler H.C, & Suter U. (2018). De novo fatty acid synthesis by Schwann cells is essential for peripheral nervous system myelination. The Journal of Cell Biology, 217(4), 1353-1368.

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