An EdU assay was performed using the Cell Light EdU DNA imaging kit (Guangzhou RiboBio Co., Ltd.)to measure the effects of let-7g/i on cellular proliferation. The EdU assay was performed 48 h after cells were transfected with let-7g/i agomir. Cells were seeded in 96-well plates and exposed to 25 mm EdU for 2 h at 37°C, and were then fixed in 4% paraformaldehyde. Following permeabilization with 0.5% Triton X-100 (Amresco LLC, Solon, OH, USA), the 16 Apollo reaction cocktail (Guangzhou RiboBio Co., Ltd.) was added and the cells were incubated for 30 min. Subsequently, the DNA of the cells was stained with Hoechst 33342 (Sigma-Aldrich, St. Louis, MO, USA) for 30 min and visualized under a fluorescent microscope (IX81; Olympus Corporation, Tokyo, Japan). The cell count was analyzed by Image-Pro Plus 6.0 software (Media Cybernetics, Inc., Rockville, MD, USA).
16 apollo reaction cocktail
Manufactured by RiboBio
Sourced in China
The 16 Apollo reaction cocktail is a laboratory reagent used in molecular biology and genetics research. It serves as a premixed solution containing essential components for performing reverse transcription and polymerase chain reaction (RT-PCR) experiments. The specific composition and formulation of the cocktail are designed to facilitate efficient and reliable nucleic acid amplification.
Automatically generated - may contain errors
Lab products found in correlation
Cell light edu dna imaging kit, by RiboBio (2 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by RiboBio (1 mentions)
5 ethynyl 2 deoxyuridine edu, by RiboBio (1 mentions)
Tcs sp5, by Leica (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Mr 96a, by Mindray (1 mentions)
3 protocols using 16 apollo reaction cocktail
1
EdU Assay for Measuring Cell Proliferation
2
Quantifying Cell Proliferation via EdU Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An EdU assay was performed using the Cell Light EdU DNA imaging kit (Guangzhou RiboBio Co., Ltd., Guangzhou China) to measure cell proliferation. 48 hours after transfection, cells were seeded in 96-well plates and exposed to 25 mM EdU for 2 h at 37°C, and were then fixed in 4% paraformaldehyde. Following permeabilization with 0.5% Triton X-100, the 16 Apollo reaction cocktail (Guangzhou RiboBio Co., Ltd., Guangzhou China) was added and the cells were incubated for 30 min. Subsequently, the DNA of the cells was stained with 4',6-diamidino-2-phenylindole (DAPI) (Guangzhou RiboBio Co., Ltd., Guangzhou China) for 15 min and visualized under a fluorescent microscope (IX81; Olympus Corporation, Tokyo, Japan). The cell count was analyzed.
3
MTT and EdU Assays for Cell Viability and Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For methyl thiazolyl tetrazolium (MTT) (Sigma, St. Louis, MO, USA) colorimetric assay, cells were seeded in 96-well plates with 2×103 cells/well. Next, 20 mL aliquot of MTT (5 mg/mL) was applied per well for 4-h incubation, followed by solubilization of formazan precipitate in 150 mL of dimethylsulfoxide (DMSO, Sigma, St. Louis, MO, USA). A microplate reader (MR-96A, Mindray, Shanghai, China) was used for recording the absorbance at 490 nm. For the EdU assay, cells were subjected to 25 µM 5-ethynyl-2'-deoxyuridine (EdU, RiboBio, Guangzhou, China) exposure for 2 h at 37 °C, with 4% PFA (RiboBio) fixation and 0.5% Triton-X (RiboBio) permeabilization. After incubation with 16 Apollo reaction cocktail (RiboBio) for 30 min, nuclei were subjected to 30-min dye with 4',6-diamidino-2-phenylindole (DAPI, Bepharm, Ltd. Shanghai, China) and captured under laser scanning confocal microscopy (TCS Sp5, Leica, Germany). All experiments were performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!