1 phenyl 1 2 ethanediol

Styrene oxide (racemic), 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), 4-nitrophenyl acetate (NPA), and 1-phenyl-1,2-ethanediol were purchased from Sigma-Aldrich (Steinheim, Germany). Reduced l-glutathione (GSH) and oxidized glutathione (GSSG) were ordered from Carl Roth (Karlsruhe, Germany).
The plasmids pET16bp_StyI and pET16bp_StyJ for the recombinant expression of the glutathione S-transferases carry the respective genes in a codon-optimized form synthesized by Eurofins MWG (NCBI GenBank accession numbers are MW590822 for styI and MW590823 for styJ). They were inserted into the multiple-cloning site (MCS) of pET16bp via the NdeI and NotI restriction sites, as described earlier for other genes (22 (link)). Correct insertion of constructs was checked via sequencing. Resulting expression products will carry an N-terminal His₁₀ tag.

Styrene oxide (racemic), 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB), 4-nitrophenyl acetate (NPA), and 1-phenyl-1,2-ethanediol were purchased from Sigma-Aldrich (Steinheim, Germany). Reduced l-glutathione (GSH) and oxidized glutathione (GSSG) were ordered from Carl Roth (Karlsruhe, Germany).
The plasmids pET16bp_StyI and pET16bp_StyJ for the recombinant expression of the glutathione S-transferases carry the respective genes in a codon-optimized form synthesized by Eurofins MWG (NCBI GenBank accession numbers are MW590822 for styI and MW590823 for styJ). They were inserted into the multiple-cloning site (MCS) of pET16bp via the NdeI and NotI restriction sites, as described earlier for other genes (22 (link)). Correct insertion of constructs was checked via sequencing. Resulting expression products will carry an N-terminal His₁₀ tag.