Eyes from one animal were used for immunohistochemistry. Dissection and fixation were performed as previously described [13 (link)]. Hematoxylin and eosin staining was performed as described by Kiernan [52 (link)]. Anti-bodies used for staining included a polyclonal IgG antibody directed at IL-18 (MBS2026569, MyBioSource, San Diego, CA, USA), a polyclonal IgG antibody directed at S100A12 (MBS2026249, MyBioSource, San Diego, CA, USA), a polyclonal IgG antibody directed at ANXA1 (MBS2001804, MyBioSource, San Diego, CA, USA), a poly-clonal IgG antibody directed at fibronectin (ab23751, Abcam, Cambridge, UK), and a monoclonal IgG1 antibody directed at rhodopsin (ab190307, Abcam, Cambridge, UK). The antibodies were diluted in (1:200–1:800) in PBS + 0.3% Triton X100. The sections incubated overnight at 4 °C and were processed with EnVission (DakoCytomation) DAB. Controls were incubated with rabbit IgG2b or irrelevant rabbit anti-bodies.
Envission
Manufactured by Agilent Technologies
Sourced in Denmark
The EnVision is a high-performance multimode microplate reader from Agilent Technologies. It is designed to measure a wide range of assays, including absorbance, fluorescence, and luminescence, in a variety of microplate formats. The EnVision provides reliable and accurate results, with advanced optics and detection technologies to ensure consistent performance.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using envission
1
Immunohistochemical Analysis of Eye Tissues
2
Immunostaining of Retinal BRVO Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eyes from one animal were used for immunohistochemistry to compare BRVO + bevacizumab (n=1) to BRVO + NaCl (n=1). Immunohistochemical analyses were performed on retinal sections containing the occlusions. Fixation was performed using a fixative of 99% ethanol (three parts) and glacial acetic acid (one part) as previously described [4 (link)]. A polyclonal IgG antibody directed at TTR (ab9015, Abcam) was used for immunohistochemistry. Sheep antibodies were diluted (1:200 to 1:800) in PBS (1X; 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4) with 0.3% Triton X100. Retinal sections were incubated overnight at 4 °C and were processed using EnVission (DakoCytomation, Glostrup, Denmark) DAB. Controls were incubated with either sheep IgG2b alone or irrelevant sheep antibodies.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!