Pregnant wild-type CD-1 female mice or wild-type CD-1 litters were obtained from Charles River. The litter of one female (10–16 mice) was sacrificed at seven days (P7) for GNP isolation. All procedures were approved by the Institutional Animal Care and Use Committee in accordance with established animal care guidelines.
Wild type cd 1 female mice
Manufactured by Charles River Laboratories
Sourced in United States
Wild-type CD-1 female mice are a commonly used mouse strain in biomedical research. They are outbred and possess a normal genetic background, allowing for the study of diverse biological processes.
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Lab products found in correlation
2 protocols using wild type cd 1 female mice
1
Isolating Postnatal Mouse GNPs
2
Isolation of Murine Kidney Cells for scRNA-seq
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Timed pregnant wild-type CD-1 female mice used in this study were obtained from Charles River Laboratories (Wilmington, MA, USA). The date on which the plug was observed was considered embryonic day 0.5 (E0.5). All experimental procedures were performed in accordance with the University of Pittsburgh Institutional Animal Care and Use Committee guidelines (IACUC protocol #17091432), which adheres to the NIH Guide for the Care and Use of Laboratory Animals.
We harvested two kidneys at E14.5 and generated a single cell suspension using 0.05% trypsin at 37°C for 10 minutes. Kidneys were mechanically dissociated with pipetting at 5 and 10 min. 3% fetal calf serum in PBS was added to halt the trypsin. The cell suspension was filtered using a 40 µm filter and pelleted. The cells were resuspended in 90% FCS in DMSO and frozen, prior to shipment to GENEWIZ Ing. Single-cell library preparation and sequencing was performed by GENEWIZ Inc. using the 10X Genomics Inc. Chromium 3' Single Cell v2 library preparation kit. Cells exhibited high viability after freezing and thawing (>90%).
We harvested two kidneys at E14.5 and generated a single cell suspension using 0.05% trypsin at 37°C for 10 minutes. Kidneys were mechanically dissociated with pipetting at 5 and 10 min. 3% fetal calf serum in PBS was added to halt the trypsin. The cell suspension was filtered using a 40 µm filter and pelleted. The cells were resuspended in 90% FCS in DMSO and frozen, prior to shipment to GENEWIZ Ing. Single-cell library preparation and sequencing was performed by GENEWIZ Inc. using the 10X Genomics Inc. Chromium 3' Single Cell v2 library preparation kit. Cells exhibited high viability after freezing and thawing (>90%).
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